Jan 17, 2011 · In this paper we describe a semiautomatic Cryo-Electron Microscopy image analysis framework to facilitate biophysical analysis of lipid nanoparticles carrying siRNA for in vivo therapeutics.

Cryo-TEM studies show that these LNP siRNA systems exhibit an electron-dense core (in contrast to bilayer vesicle systems), and that “limit size” systems can be generated at high PEG-lipid contents that are consistent with the ability of siRNA to stimulate formation of inverted micelles in association with cationic lipid.

Jan 17, 2011 · We apply this approach to characterize two formulations of siRNA-loaded lipid nanoparticles composed of cationic lipid, cholesterol, and poly (ethylene glycol)-lipid, where the formulations differ only by input component ratios.

Jul 20, 2021 · Here, we show that a permeating cationic dye, thionine, can serve as a cryogenic electron microscopy contrasting agent by binding selectively to encapsulated mRNA without disturbing lipid nanoparticle morphology.

Apr 3, 2018 · Here we provide cryogenic transmission electron microscopy (cryo-TEM) and X-ray evidence that the complexes formed between siRNA and ionizable lipid at pH 4 correspond to tightly packed bilayer structures with siRNA sandwiched between closely apposed monolayers.

Sep 28, 2020 · A new cationic-lipid/siRNA particle that was designed to deliver siRNA was investigated by the combination of small-angle X-ray scattering (SAXS), asymmetric field flow fractionation coupled with multiangle light scattering, and …

Oct 27, 2020 · A new cationic-lipid/siRNA particle that was designed to deliver siRNA was investigated by the combination of small-angle X-ray scattering (SAXS), asymmetric field flow fractionation coupled with multiangle light scattering, and …

Cryo-TEM is a technique used to visualize the structure of frozen and fully hydrated macromolecular complexes. The sample is studied at cryogenic temperatures (generally liquid nitrogen temperatures) and no fixation or staining of the sample is required.

The cryo-TEM image of the primary emulsion ( Figure 7 A) showed aggregated lipid droplets and a distinct electrically dense siRNA-ionizable lipid complex with insufficient encapsulation.

Sep 1, 2024 · A multilamellar nanostructured core was formed inside the nucleic acid-loaded LNP, which was visualized by cryogenic transmission electron microscopy (cryo-TEM) [14, 23].