Genome-wide screening reveals a role for subcellular ... - Nature
Mar 4, 2020 · CRBN-knockout cells were obtained from OPM-2 and 293T cells by transfecting a pX330-based CRISPR/Cas9 vector 30 targeting the first exon of CRBN, and treating the cells …
Discovery of First-in-Class PROTAC Degraders of SARS-CoV-2 …
Apr 12, 2024 · MPD2 effectively reduced M Pro protein levels in 293T cells, relying on a time-dependent, CRBN-mediated, and proteasome-driven mechanism. Furthermore, MPD2 …
CRBN Knockout cell line(293T) - Ubigene
The CRBN Knockout cell line(293T) is constructed based on (1) the optimal design of the Red Cotton System, (2) CRISPR-U™ technology with efficiency 10-20 times higher than traditional …
Discovery of novel potential CRBN modulators through structure …
Dec 1, 2022 · CRBN protein is an E3 ubiquitin ligase which plays an important role in the ubiquitin-proteasome system of eukaryotic cells. Small molecules can modulate CRBN and …
Lenalidomide Causes Selective Degradation of IKZF1 and IKZF3 in ...
Nov 28, 2013 · Western blots of 293T cells lysates 48 hours after cotransfection of FLAG-tagged IKZF3 or IKZF4 with HA-tagged CRBN and 24 hours of drug treatment.
A novel cereblon modulator recruits GSPT1 to the CRL4 CRBN …
Jun 22, 2016 · Immunomodulatory drugs bind to cereblon (CRBN) to confer differentiated substrate specificity on the CRL4 CRBN E3 ubiquitin ligase. Here we report the identification …
Cereblon attenuates DNA damage-induced apoptosis by …
Jan 25, 2019 · Human embryonic kidney (HEK) 293T cells were first transfected with siNC or siCRBN for 24 h and then co-transfected again with GFP or GFP-p53 and Flag-Bcl-2 (c) or …
Degradation-Based Protein Profiling: A Case Study of Celastrol
Apr 25, 2024 · Flag-CRBN or Flag-DCAF16 lentivirus was generated by co-transfection of Flag-CRBN or Flag-DCAF16, psPAX2 and pMD2.G into 293T cells using Lipofectamine 3000 …
Glutamine triggers acetylation-dependent degradation of …
Mar 3, 2016 · Cereblon (CRBN), a substrate receptor for the cullin–RING ubiquitin ligase 4 (CRL4) complex, is a direct protein target for thalidomide teratogenicity and antitumor activity …
The novel cereblon modulator CC-885 inhibits mitophagy via …
As CC-885-induced protein degradation was dependent on cereblon (CRBN) , we then attempted to identify the degraded proteins in our list that were regulated by CRBN. Toward this goal, we …