In this study, we have expressed Enhanced Green Fluorescent Protein (EGFP)–tagged Rab7 wt as well as active and dominant-negative mutant proteins and analyzed their effect on the localization and other properties of late endocytic structures by confocal and electron microscopy.

Oct 13, 2017 · In this study, we have expressed Enhanced Green Fluorescent Protein (EGFP)–tagged Rab7 wt as well as active and dominant-negative mutant proteins and analyzed their effect on the localization and other properties of late endocytic structures by confocal and electron microscopy.

Nov 3, 2020 · Loss of the Rab7-NPC1 interaction in MCC-deficient cells correlates with lysosomal cholesterol accumulation, suggesting Rab7 activation is essential for NPC1-dependent lysosomal cholesterol...

Lacking from previous work is an examination of Rab7 and LAMP1 simultaneously. Using confocal fluorescence microscopy to image Rab7 and LAMP1, we find that the majority (70%) of endo-lysosomal vesicles are positive for both Rab7 and LAMP1.

Jul 14, 2014 · Alonso-Curbelo et al. identify a unique dependency in melanoma on the small GTPase RAB7 due to regulation by both oncogenic and lineage-specific factors. RAB7 controls melanoma cell phenotype and represents a possible vulnerability that …

Acquisition of Rab7 and its effector protein RILP to the SCV were studied using confocal fluorescence microscopy. Because available antibodies are inadequate to detect the endogenous proteins by immunostaining, we transfected HeLa cells with GFP-tagged chimeric constructs of …

Jan 15, 2016 · A combination of a Rab7 sensor and confocal FRET imaging techniques revealed that the activation of Rab7 on late endosomes depends on Mon1–Ccz1 and is implicated in late-endosome–lysosome fusion. In contrast, Rab7 activity on lysosomes was independent of Mon1–Ccz1 and active Rab7 played a role in perinuclear clustering of lysosomes.

Oct 30, 2001 · We and others have identified a protein, called RILP (for Rab7-interacting lysosomal protein), that interacts with active Rab7 on late endosomes and lysosomes. Here we show that RILP prevents further cycling of Rab7.

RAB7 overexpression in Cd19+/creRosa26fl-STOP-fl-Rab7 mouse B cells upregulates K63 polyubiquitination activity of TRAF6, enhances NF-κB activation and AID induction, and boosts IgG antibody and autoantibody levels.

Nov 20, 2017 · Confocal analysis of endogenous RAB7 and LAMP2 revealed a pronounced increase in co‐localization in VPS29 CRISPR‐transfected cells which were used as positive control in our screen (Fig 3A).