Jun 19, 2017 · With the goal of modeling human disease of the large intestine, we sought to develop an effective protocol for deriving colonic organoids (COs) from differentiated human embryonic stem cells...

As for colonic organoids, we have recently reported the successful generation of HCOs through the direct differentiation of hPSCs, in a manner that was similar to HIOs, but with important modifications as described below.

Aug 1, 2024 · Here, we report the generation of bioengineered human colon and small intestinal organoids-on-chip that exhibit remarkable functional and morphological resemblance to the native tissue and are suitable for a variety of biological and translational applications.

Aug 11, 2021 · Injection of intestinal organoids allows coculture under the most physiological conditions, but standardization and scalability of this protocol is difficult to achieve. Below, we provide a...

Nov 25, 2019 · Colonic organoids are comprised of all major types of colonic epithelial cells and represent the architecture and function remarkably similar to those of the colonic epithelium, faithfully recapitulating the functional colonic epithelium ex vivo.

May 31, 2024 · Intestinal organoids are favored in drug screening due to their ability for high-throughput in vitro cultivation and their closer resemblance to patient genetic characteristics.

Mar 20, 2025 · Colon organoids provide a three-dimensional (3D) system that captures key structural and functional characteristics of the intestinal epithelium, enabling more representative studies of disease progression, drug absorption, and tissue regeneration.

Gastric and small intestinal organoids differentiated from human pluripotent stem cells (hPSCs) have revolutionized the study of gastrointestinal development and disease. Distal gut tissues such as cecum and colon, however, have proved considerably more challenging to derive in vitro.

Aug 1, 2024 · Organoids and organs-on-a-chip have emerged as powerful tools for modeling human gut physiology and disease in vitro. Although physiologically relevant, these systems often lack the environmental milieu, spatial organization, cell type diversity, and maturity necessary for mimicking human intestinal mucosa.

Human intestinal organoids (HIOs) derived from human pluripotent stem cells co-differentiate both epithelial and mesenchymal lineages in vitro but lack important cell types such as neurons, endothelial cells, and smooth muscle, which limits translational potential. Here, we demonstrate that the intestinal stem cell niche factor, EPIREGULIN (EREG), …