Co-IP experiments can identify proteins via direct or indirect interactions or in a protein complex. Here, we use Agrobacterium type VI secretion system (T6SS) sheath components TssB-TssC 41 interaction as an example to describe the principle, procedure, and experimental problems of …

Co-immunoprecipitation (co-IP) of protein complexes from cell lysates is widely used to study protein-protein interactions. However, establishing robust co-IP assays often involves considerable optimization. Moreover, co-IP results are frequently presented in …

Here, we describe a co-immunoprecipitation protocol to study PPIs between endogenous nuclear proteins under hypoxic conditions. The interaction between HIF-1α and ARNT is shown as a proof of concept.

Protein-protein interactions (PPIs) are the physical contacts between two or more proteins and they represent complex biological functions. Nowadays, PPIs have been used to construct PPI networks to study complex pathways for revealing the functions of unknown proteins.

Co-immunoprecipitation (co-IP) is a popular technique to identify physiologically relevant protein–protein interactions by using target protein-specific antibodies to indirectly capture proteins that are bound to a specific target protein.

Part 1 is a simple coIP method for directed coIP assays, to determine whether two known proteins (or a protein and RNA) are found in a complex. Part 2 is an elaboration of this method for the purification of complexes for identification of unknown components by mass spectrometry.

Co-immunoprecipitation (Co-IP) is a technique to identify protein-protein interactions. Proteins often do not function as single substances but rather as team players in a dynamic network. Growing evidence shows that protein–protein interactions are crucial in many biological processes in living cells. Genetic (such as yeast two-hybrid, … more.

Jul 1, 2017 · Co-IP experiments can identify proteins via direct or indirect interactions or in a protein complex. Here, we use Agrobacterium type VI secretion system (T6SS) sheath components TssB-TssC 41 interaction as an example to describe the principle, procedure, and experimental problems of co-IP.

Mar 15, 2022 · Co-immunoprecipitation coupled with mass spectrometry (Co-IP/MS) is an extremely powerful analytical technique and has been pivotal in PPI studies to identify protein complexes, co-factors and signaling molecules [3, 4].

Oct 28, 2010 · Analysis of protein-protein interactions (PPIs) is a valuable approach for the characterization of huge networks of protein complexes or proteins of unknown function. Co-immunoprecipitation (coIP) using affinity resins coupled to protein A/G is the most widely used method for PPI detection.