F430 is the cofactor (sometimes called the coenzyme) of the enzyme methyl coenzyme M reductase (MCR). [1][2] MCR catalyzes the reaction EC 2.8.4.1 that releases methane in the final step of methanogenesis: It is found only in methanogenic Archaea [3] and anaerobic methanotrophic Archaea.

Coenzyme F430 is a nickel tetrapyrrole found only in methanogenic Archaea. It is the prosthetic group of the enzyme methyl coenzyme M reductase which catalyses the release of methane in the final step of methanogenesis.

Feb 22, 2017 · The enzyme uses an ancillary factor called coenzyme F430, a nickel-containing modified tetrapyrrole that promotes catalysis through a methyl radical/Ni (ii)-thiolate intermediate.

Oct 21, 2016 · We used comparative genomics to identify the coenzyme F430 biosynthesis (cfb) genes and characterized the encoded enzymes from Methanosarcina acetivorans C2A. The pathway involves nickelochelation by a nickel-specific chelatase, followed by amidation to form Ni-sirohydrochlorin a, c -diamide.

Dec 11, 2017 · Methanogens perform the crucial final step in methane production, and monitoring their activity is of paramount importance for system understanding and management. Cofactor F 430 is an essential prosthetic group of the methyl-coenzyme M reductase (MCR) enzyme catalysing this final step.

Coenzyme F430 is a nickel-containing tetrapyrrole that is required by methyl-coenzyme M reductase (Figure 29). It is one of a class of nickel chlorins found in nature.148 Methyl-coenzyme M reductase catalyzes the last step in methanogenesis, the reduction of methyl-coenzyme M to methane and coenzyme M (step 8, Figure 1).

Apr 12, 2023 · Methyl-coenzyme M reductase, responsible for the biological production of methane by catalyzing the reaction between coenzymes B (CoBS-H) and M (H 3 C-SCoM), hosts in its core an F430 cofactor with the low-valent Ni I ion.

The work introduces the use of cofactor F 430 as a biomarker for methanogenic activity and, hence, as a monitoring tool that can be quantified within half a working day, yielding information directly related to in situ methanogenic activity in methanogenic reactors.

Feb 11, 2022 · Methyl-coenzyme M reductase (MCR) containing a nickel hydrocorphinoid cofactor, F430, is an essential enzyme that catalyzes anaerobic methane generation and oxidation. The active Ni (i) species in MCR converts methyl-coenzyme M (CH3S–CoM) and coenzyme B (HS–CoB) to methane and heterodisulfide (CoM–S–S–CoB).

Bending of the reduced porphyrin of factor F430 can accommodate a trigonal-bipyramidal geometry at nickel: A conformational analysis of this nickel-containing tetrapyrrole, in relation to archaebacterial methanogenesis.