Apr 26, 2021 · Conditional knockout (cKO) mice are genetically engineered animal models in which certain gene is inactivated in a specific tissue or on an inducible basis. Researchers can understand certain gene functions through time or tissue specific deletion to study the mechanisms of human disease.

What is a conditional knockout mouse? Conditional knockout (cKO) mouse systems rely on a classical genetic recombination tool called Cre-loxP, derived from bacteriophage P1 . Cre is an enzyme that recombines loxP sites and deletes the DNA segment between the two loxP sites.

KO mice are typically generated by deleting a gene from the mouse genome using gene targeting techniques such as homologous recombination or CRISPR/Cas9 genome editing. In contrast, cKO mice are generated using the Cre-loxP system, which allows for precise control of gene expression in specific tissues or at specific developmental stages.

Researchers use cKO mouse models to bypass limitations of constitutive knockout strategies, such as embryonic lethality, modified animal physiology, compensation mechanisms, and more.

Oct 19, 2020 · We are excited to announce our newest upgrade to the Cyagen Knockout Catalog Models repository, which now includes conditional knockout (cKO) mouse models – also known as floxed mice. Our searchable database now features 300+ cKO/floxed mouse strains – with cohorts available for as low as $18,999.

Therefore, there has been a need for genetically engineered mice in which only astrocytic MAOB is targeted. Here, we generated an astrocyte-specific MAOB conditional KO (cKO) mouse line and characterized it in the cerebellar and striatal regions of the brain.

Apr 27, 2021 · Conditional knockout (cKO) mice can be used to study specific gene functions and related human diseases. Compared with traditional knockout (KO) mice, conditional knockout mice are obtained with a gene that deleted only in specific tissues or on an inducible basis.

Apr 14, 2020 · Thus, we developed a forebrain excitatory neuron-specific SENP2 knockout mouse model to examine SENP2 functions in the CNS. We found that these conditional knockout (cKO) animals display hyperactivity and reduced anxiety-like behavior, impaired learning and memory.

In conditional knockout mice, a gene is inactivated in a specific tissue to study the function of individual genes and model human diseases. Our TurboKnockout ® service can provide you with knockout and conditional knockout (flox) mouse models in as fast as 6-8 months.

Conditional knockout (cKO) mice are extremely valuable for biomedical research because they enable detailed analyses of gene functions in a tissue- or temporally-specific fashion.