CITE-seq uses DNA-barcoded antibodies to convert detection of proteins into a quantitative, sequenceable readout. Antibody-bound oligos act as synthetic transcripts that are captured …

Below are our main protocols for performing CITE-seq and Cell Hashing, specifically on Drop-seq or 10x Genomics single cell 3P chemistry (v2 and v3), followed by supplementary information …

CITE-seq antibody-oligos (ADTs): These contain standard small TruSeq RNA read 2 sequences and can be amplified using Illumina’s Truseq Small RNA primer sets (RPIx – primers, see …

Our ECCITE-seq paper describing the capture of multiple modalities of information from single cells, including direct capture of guide RNAs (Nature Methods, online April 22, 2019). You can …

Can I stain samples with Cell Hashing and/or CITE-seq and our fluorescent panel at the same time? Yes. We often first enrich cell populations by FACS and then run CITE-seq or multiplex …

Rahul Satija’s lab (NYGC & NYU) has developed tools for read level processing of CITE-seq data (listed below) and has recently update their Seurat package to support multimodal data. You …

We have adapted CITE-seq and Cell Hashing to be compatible with the 5P / V(D)J single cell kit from 10x Genomics, to allow researchers to perform sample multiplexing, doublet detection …

Apr 22, 2019 · 10-16-17: Rahul Satija and Peter Smibert awarded one of 38 pilot projects for the Human Cell Atlas, funded by Chan Zuckerberg Initiative, benchmarking CITE-seq for immune …

CITE-seq & Cell Hashing Protocol For experiments involving Cell Hashing, use cost per cell calculator to plan experiments, determine number of hashes, number of cells to load, expected …

Dec 21, 2018 · Cell Hashing is a method that enables sample multiplexing and super-loading on single cell RNA-sequencing platforms, developed in the Technology Innovation lab at the New …