Here, we report the 11-angstrom–resolution cryogenic electron microscopy (cryo-EM) structures of 30-nanometer chromatin fibers reconstituted in the presence of linker histone H1 and with different nucleosome repeat lengths.

One chromatin remodeling mechanism called “nucleosome sliding” allows a nucleosome to be “moved” to control gene access, but how this works has been incompletely understood. To address this gap, Mario Halic, PhD, St. Jude Department of Structural Biology, used cryo-EM to capture the chromatin remodeling enzyme SNF2H in action.

4 days ago · To better understand how chromatin remodeling works, scientists at St. Jude Children's Research Hospital used cryo-electron microscopy (cryo-EM) to obtain fine structural details of a human ...

Apr 25, 2014 · Here, we report the 11-angstrom-resolution cryogenic electron microscopy (cryo-EM) structures of 30-nanometer chromatin fibers reconstituted in the presence of linker histone H1 and with different nucleosome repeat lengths.

Oct 1, 2022 · Cryo-EM technology captures snapshot structures of molecular machines, such as RNA polymerase II transcribing nucleosomal DNA. Since chromatin structures are deeply connected to nuclear functions, cryo-EM analyses of native chromatin segments are important to clarify genome functions.

Recently, a variety of chromatin structures have been determined by single-particle cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET), and their structural details have provided clues about the chromatin architecture functions in the cell.

Apr 3, 2025 · Chromatin remodelers utilize the energy of adenosine triphosphate (ATP) hydrolysis to slide nucleosomes, regulating chromatin structure and gene activity in cells. ... Cryo-EM data were acquired with the defocus set in the range of -1.4 to -1.8 μm, and at a nominal magnification of 81,000x, which renders a final pixel size of 0.54125 Å/pixel.

Apr 3, 2025 · To better understand how chromatin remodeling works, scientists at St. Jude Children’s Research Hospital used cryo-electron microscopy (cryo-EM) to obtain fine structural details of a human ...

Oct 1, 2020 · This review will focus on cryo-electron microscopy (cryo-EM) studies that relate to regular chromatin fibers with a periodic structure (Table 1). For studies of the irregular, more dynamic chromatin structures observed in many cells, readers are referred to a …

Mar 4, 2024 · In this research, we aimed to investigate the molecular mechanism underlying the coupling of ATP hydrolysis to chromatin remodelling by DDM1 using structural biology approaches.