Mar 16, 2022 · Experimentally naive C57BL/6J mice received an AAV-hSyn-ChR2 injection into the mPFC and an optic-fiber implantation at one of the downstream regions (Fig. 4a, b). They quickly learned to...

Jul 22, 2020 · For optical tagging of PV- or SST-expressing INs, we delivered Cre-inducible adeno-associated virus (AAV) carrying the gene for channelrhodopsin-2 (ChR2-mCherry) into the mPFC of PV-Cre (20) or SST-Cre (21) knock-in mice, respectively.

Jan 15, 2019 · A Cre-dependent channel rhodopsin viral vector (AAV-Chr2) was used to selectively express Chr2 in the mPFC of Drd1-and Drd2-Cre recombinase mice, resulting in Chr2-EYFP fluorescence in the ...

Dec 23, 2022 · Photostimulation activated ChR2+ mPFC engram inputs and induced EPSCs in tdT− nonengram and tdT+ engram BLA neurons.

Photostimulation of the vmPFC was achieved using a herpes simplex virus (HSV) viral vector coding for ChR2 driven by the IE4/5 promoter, which targeted ChR2 to mPFC neurons in a non-selective manner (Covington et al., 2010).

May 5, 2018 · To investigate the contribution of the mPFC to flexible choice behavior, we used VGAT-ChR2-EYFP transgenic mice, previously used for precise inactivation of small volumes of cortical tissue by photostimulation of ChR2 in GABAergic interneurons (Zhao et al., 2011; Guo et …

Thus, ChR2-mediated activation of mPFC axons leads to an action potential-dependent and Ca 2+ channel-dependent glutamate release. In the presence of TTX, light illumination induced glutamate release from PFC axons onto 5-HT neurons, but only provided that the ChR2-mediated depolarization of isolated axons was unabated by the activation of K ...

Oct 8, 2012 · Confocal microscopy confirmed ChR2-EYFP or control vector expression in mPFC pyramidal cells but not in GABAergic cells. The novelty of our study is the analysis of optogenetic effects on background and evoked activity of defined cell types in different mPFC regions.

Jul 7, 2020 · Somatic eYFP expression was used to identify ChR2 positive neurons in the mPFC under cell-attached mode to assess the efficiency of optogenetic drive. For cell-attached experiments, pipettes (4–6 MΩ) were filled with ACSF.

Dec 12, 2013 · To explore synaptic mechanisms of fear extinction, we trained mice expressing ChR2 in the mPFC in the auditory fear conditioning paradigm (see Experimental Procedures; Figure 2A).