Efficient C•G-to-G•C base editors developed using CRISPRi screens ...
Jun 28, 2021 · We demonstrate a machine learning workflow using CGBE-Hive to identify optimal CGBE and sgRNA editing strategies to install a desired edit, and show that this workflow …
Nat Biotech背靠背 | 单碱基编辑新工具可实现C-G及C-A碱基颠换
近年来研究者通过开发 单碱基编辑工具 CBE（Cytosine Base Editor）和 ABE （Adenine Base Editor）分别实现了C --T及A--G碱基的自由转换【2，3】，这对众多因碱基转换导致的遗传病 …
Glycosylase-based base editors for efficient T-to-G and C-to-G …
Jan 2, 2024 · In this study, we constructed deaminase-free base editors for cytosine (DAF-CBE) and thymine (DAF-TBE), which contain only a cytosine-DNA or a thymine-DNA glycosylase …
CRISPR C-to-G base editors for inducing targeted DNA ... - Nature
Jul 20, 2020 · We show that CGBE1 can efficiently induce C-to-G edits, particularly in AT-rich sequence contexts in human cells. We also removed the eUNG domain to yield miniCGBE1, …
CRISPR 101: Cytosine Transversion Editors - blog.addgene.org
BE4max, a CBE, generated similar deletions at a lower frequency. Their data suggest that DSBs are a common intermediate of CGBE activity, and they are sometimes processed by error …
Developing a highly efficient CGBE base editor in watermelon
Jul 23, 2023 · To enhance the applicability of base editing technology in watermelon, we developed an efficient CGBE editor (SCGBE2.0) by removing the uracil glycosylase inhibitor …
C-G Base Editor - 知乎 - 知乎专栏
目前常见的单碱基BE（Base Editor）工具包括CBE（Cytosine ）和ABE（Adenine Base Editor），其通过 脱氨反应 实现了碱基的转换（transition）。 然而， 嘧啶 （pyrimidine）向 …
Developing safe and efficient CGBE editor based on Cas …
CGBE (C-to-G base editor) systems, pivotal components within the base editing arsenal, enable the precise conversion of cytosines to guanines. However, conventional cytidine deaminases …
Development of a set of C•G-to-G•C transversion base editors …
We provide trained CGBE-Hive machine learning models which accurately predict CGBE efficiency, C•G-to-G•C editing purity, and bystander editing patterns (R=0.90) to enable …
Nature子刊：杨辉团队开发新型DNA碱基编辑器，首次实现高效腺 …
Jan 14, 2023 · 在CBE基础上开发出的CGBE或GBE（Glycosylase base editor，糖基化酶碱基编辑器）能实现C-to-G和C-to-A此类碱基之间的颠换（transversion；即嘧啶变嘌呤）。 CGBE …