Treg suppression can be measured in these assays by co-culturing CFSE-labeled Teff (target cells) in the presence of Tregs (suppressor cells) and monitoring inhibition of proliferation and activation of target cells .

Apr 30, 2007 · The analysis of Treg function in patients with autoimmune, allergic or oncogenic diseases has emerged over the past years. In the present study we describe a CFSE based protocol to measure Treg mediated suppression of CD4(+) T cells.

Herein we describe our work utilizing CFSE labeling to assess, (1) proliferative responses of CD4 (+) and CD8 (+) T-cells in unseparated single cell suspensions from human lymph nodes and, (2) the ability of tumor infiltrating suppressive populations, including Tregs, isolated from neoplastic lymph nodes to suppress in vitro proliferation of all...

Apr 30, 2007 · In this study we optimized a CFSE based protocol to measure Treg suppression towards both polyclonal and auto-antigen specific T cell responses and compared this assay with the traditional proliferation based assay to measure Treg activity.

Feb 3, 2025 · In this head-to-head comparison, we find Tregs having the clearest suppressive effects on effector memory CD8 T cells through partial division arrest, cell cycle inhibition, and effector molecule...

CFSE labeling of responder cells allows Treg cells and responding cells to be assayed independently and also permits analyses in parallel with proliferation, such as cell-surface marker expression or cytokine production of proliferating versus nonproliferating (suppressed) responders 23.

CFSE-based co-culture has become the gold standard for proliferation assays and has been used successfully to assess the function of Tregs. Here, based on CFSE co-culture assays, we show that the in vitro -expanded CD8 + CD28 - Tregs maintain allospecific suppressive capacity both in vitro (Figure 1) and in vivo. Figure 1.

In this report, we show extensive fine tuning of a CFSE-based suppression assay to determine the induction of human tolerogenic DC-induced Treg. The suppression assay uses mDC-stimulated T-cell proliferation as read-out through which it becomes broader applicable, e.g. to detect suppressive mechanisms that are APC dependent and to determine ...

FOXP3+ T-regulatory (Treg) cells have important roles in immune homeostasis, and alterations in their number and function can predispose to diseases ranging from autoimmunity to allograft rejection and tumor growth. Reliable identification of human ...

Apr 30, 2007 · In this study we optimized a CFSE based protocol to measure Treg suppression towards both polyclonal and auto-antigen specific T cell responses and compared this assay with the traditional proliferation based assay to measure Treg activity.