As T cell activation leads to downregulation of T cell receptor (TCR) and coreceptor CD8, we developed a novel FACS-based sorting method to enrich activated antigen-specific CD8 + T cells.

The 53-6.7 monoclonal antibody specifically binds to the 38 kDa α and 34 kDa α' chains of the CD8 differentiation antigen (Ly-2 or Lyt-2) of all mouse strains tested. The CD8 α and α' chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly …

We observed that CT-CD8a inhibited tetramer staining of mouse transgenic F5 CD8 + T-cells, whereas CT-CD8b enhanced tetramer staining of the same antigen-specific population (Figure 11A). These results are consistent with our previous findings (26).

Clone REA601 is specific for the mouse CD8a antigen, also known as LY-2 or T8, which is expressed on cytotoxic T cells and at lower levels on subpopulations of dendritic cells and TCRγ/δ+ cells. CD8a is further detected on most thymocytes (CD4+CD8a+ and …

Jan 3, 2023 · Here, we report the effectiveness of mouse CD8α+ DCs derived from bone marrow hematopoietic stem cells (BM-HSCs), equivalent to human CD141+ DCs, which have proven to be a highly superior subset....

Using FACS analysis we were able to show that in comparison the CD161−CD8α+CD8β high population, CD161−CD8α+CD8β low T cells were mostly CCR7− and CD62L− in the peripheral blood of both HC and patients with chronic HBV, HCV, and HIV-1 infections (Figures 5 A,B).

We have developed 89Zr-DFO-REGN5054, a fully human CD8A-specific antibody conjugate, to assess CD8+ tumor-infiltrating lymphocytes (TIL) pre- and posttherapy. We used multiple assays, including in vitro T-cell activation, proliferation, and cytokine production, and viral clearance and CD8 receptor occupancy, to demonstrate that REGN5054 has ...

Mar 14, 2025 · Staining and flow cytometry were then performed as described in the “Flow cytometry” section. For the memory rechallenge experiment (fig. S12), the spleens from infected mice were harvested at day 30 postinfection, and CD8 + T cells were isolated using the Miltenyi CD8a + T cell isolation kit as previously described.

Apr 15, 2001 · Discrete cytokine secretion from myeloid, lymphoid, and DN DCs from the PP and spleen. Highly purified DC subsets were obtained by FACS sorting CD11c + /MHC class II high CD11b + (myeloid), CD8α + (lymphoid), or CD8α − /CD11b − (DN) cells from PP and SP.

The percentage and absolute number of CD45.2+CFSE+cells in the lymph node (C) and T cells isolated by fluorescence-activated cell sorting (FACS) from Cre+/+Cd8a+/+ (Cd8a+/+) or Cre+/+Cd8aloxp/loxp (Cd8aloxp/loxp) mice were CFSE labeled and transferred into B6 congenic CD45.1 mice followed by tamoxifen treatment.