Aug 26, 2016 · Surface adhesion molecules [CD29 (β1-integrins), CD43, CD98, and CD147] in U937 cells (1×10 5) were analyzed by flow cytometry. All staining antibodies were used at 1~5 µg/ml. Mean fluorescence intensity (MFI, percent of control) was calculated using WIN-MDI software from a minimum of 7,500 cells.

We found that blockage of the beta 1 integrin (Itgb1/CD29) selectively hampered binding of mIgG3 to macrophages and mIgG3-mediated phagocytosis. Manganese, an integrin activator, increased mIgG3 binding to macrophages.

CD29 is widely expressed in human salivary glands and, it could serve as a potential biomarker for devising novel cellular therapeutic and diagnostic strategies for salivary gland disorders and malignancies. Keywords: Adult, CD29, fetal, progenitor, salivary gland, stem cells.

Tissino et al. demonstrate that in chronic lymphocytic leukemia, the VLA-4 (CD49d/CD29) integrin remains activable by B cell receptor stimulation also upon in vitro and in vivo ibrutinib exposure. Clinically, ibrutinib-treated CD49d-positive CLL patients experience reduced recirculation lymphocytosis and nodal response and inferior outcomes.

Mar 11, 2017 · While CD29 MFI was similar after EDTA and TT detachment, we detected a progressive 2- to 3-fold loss of surface CD29 fluorescence with increasing trypsin exposure (p < 0.05 TE2 vs. TE5; p < 0.001, TE2 and TE5 vs TT and EDTA, Fig. 3A and B).

Oct 2, 2002 · Using flow cytometric and fluorescence in situ hybridization (FISH) analysis, a high percentage of tumoral BM PC from untreated patients was found to express CD106. In addition, these cells also expressed an activated form of CD29, as determined using the CD29 activation reporter monoclonal antibody HUTS‐21.

Nov 16, 2014 · Our data showed that NK cells in aged mice have low expression of α2β1 (CD49b CD29) integrin, receptor for type I collagen with reciprocal increases in expression of α1β1 (CD49a CD29) integrin, and receptor for type IV collagen.

Aug 1, 2021 · In addition, tonsil NK cells with activated CD29 had a greater density (MFI) of activated CD29 heterodimers compared with analogous PB subsets, yet in PB, we observed a significant increase in the density of activated CD29 by MFI in stage 5 relative to PB stage 3 and 4A NK cells (Fig. 5D, Supplemental Table II).

Dec 2, 2011 · Malignant plasma cells (PC) in human multiple myeloma (MM) are retained in the bone marrow (BM) microenvironment. Using HUTS21 monoclonal antibody that reacts with active CD29 integrin, we...

Oct 12, 2011 · The carcinomas compared with mammary glands from untreated rats, showed upregulation of CD29 (Integrin β1) and CD49f expression, increased FAK (focal adhesion kinase) activation especially in the CD29hi population, and decreased CD61 (Integrin β3) expression.