Recombinant Cas9-GFP protein from Streptococcus pyogenes (~194 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, wild type Streptococcus pyogenes Cas9-GFP protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of ...

Select from Cas9 nucleases with improved editing efficiency, increased specificity, and GFP/RFP tags for visualization, always synthesized to our high quality and purity standards.

Plasmid pSpCas9(BB)-2A-GFP (PX458) from Dr. Feng Zhang's lab contains the insert hSpCas9 and is published in Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. This plasmid is available through Addgene.

Plasmid pLenti-Cas9-GFP from Dr. David Sabatini's lab contains the insert Cas9-GFP and is published in Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013. This plasmid is available through Addgene.

To enhance the binding affinity, Cas9 protein can be engineered with negatively-charged GFP or polyglutamic acid tags. Designing specific supramolecular interactions via protein engineering also contributes to resist competition from other proteins.

Recombinant enhanced specificity Cas9-GFP protein from Streptococcus pyogenes (~192 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, eSpCas9-GFP protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically ...

By directly delivering Cas9/sgRNA ribonucleoprotein (RNP) complexes through nucleofection, this protocol allows for the efficient integration of GFP 11 into a specific genomic locus via CRISPR-Cas9-mediated homology-directed repair (HDR).

wild type Streptococcus pyogenes Cas91 (Cas9-GFP Protein) or enhanced specificity Cas92 (eSpCas9-GFP Protein). Both proteins contain three varied nuclear localization sequences positioned for optimal activity. The molecular mass of Cas9-GFP Protein is 194 kDa, whereas eSpCas9-GFP Protein is 192 kDa. The EGFP

The graphs show the GFP signal versus cell count, where cell count has been normalized to the mode for cells that had either Cas9-GFP or Cas9 V3 delivered using either lipofection or Nucleofection™. (B) Alt-R CRISPR-Cas9 sgRNAs were designed to target NGG PAM sites throughout the human genome. Guides were complexed with either Cas9-GFP or Alt ...

Nov 21, 2020 · CRISPR/Cas9-mediated homology directed repair allows for fluorescent tagging of endogenous proteins in a stable hiPSC cell line, whereby a fluorescent protein such as GFP is attached to an endogenous protein of interest. By adding a small fluorescent tag, a variety of applications could be imagined.