Jul 1, 2022 · We demonstrate for the first time that cisplatin induces pyroptosis via activation of the CAPN1/CAPN2-BAK/BAX-caspase-9-caspase-3-GSDME pathway in esophageal cancer cells. These results suggest that CAPN1 and CAPN2 may represent novel biomarkers for cisplatin sensitivity in esophageal cancer.

Jul 1, 2022 · Pyroptosis-associated proteins were screened via proteomics for esophageal cancer (n = 124) and bioinformatics analysis. We observed that high calpain-1 (CAPN1) and calpain-2 (CAPN2) expression were associated with favorable clinical outcomes and prolonged survival in esophageal cancer patients.

By depleting CAPN1/CAPN2 and activating caspase-3/GSDME, cisplatin inducing pyroptosis in ESCC through CAPN1/ CAPN2-Bak/BAX-caspase-9-caspase-3-GSDMEaxis[20].STAT3 β

We observed that high calpain-1 (CAPN1) and calpain-2 (CAPN2) expression were associated with favorable clinical outcomes and prolonged survival in esophageal Cancer patients. We employed immunohistochemistry to evaluate the expression of CAPN1 and CAPN2 in pretreatment tumor biopsies from 108 patients with esophageal Cancer who received ...

The micro-array findings were replicated by the quantitative BAX and CAPN2 expression analysis using real-time PCR and by Western Blot on protein level. The biological tests (TUNEL method) for apoptosis showed consistent proapoptotic effects in all studied cell lines.

By depleting CAPN1/CAPN2 and activating caspase-3/GSDME, cisplatin inducing pyroptosis in ESCC through CAPN1/CAPN2-Bak/BAX-caspase-9-caspase-3-GSDME axis [20]. STAT3β overexpression in ESCC also promotes cisplatin-induced pyroptosis, while in SHEE cells, which are the human immortalized normal esophageal epithelial cell line here, only high ...

May 4, 2022 · Cisplatin-induced pyroptosis is mediated via the CAPN1/CAPN2-BAK/BAX-caspase-9-caspase-3-GSDME axis in esophageal cancer. According to studies, numerous chemotherapeutic drugs can facilitate programmed cell death via pyroptosis. Clarifying the mechanism by which cisplatin kills gastric cancer cells is crucial for…

May 1, 2022 · Mechanistically, we uncovered a novel mechanism whereby cisplatin induced pyroptosis via activation of a CAPN1/CAPN2-BAK/BAX-caspase-9-caspase-3-GSDME signaling axis in esophageal...

Mar 17, 2023 · Calcium-dependent calpain proteases, in particular calpain-2, are more abundant in glioblastoma compared to normal brain and increased in patient-matched initial and recurrent glioblastomas. On the cellular level, pharmacological calpain inhibition increased the sensitivities of primary glioblastoma cells towards TMZ.

Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production; Belongs to the Bcl-2 family.