Calcein, also known as fluorexon, fluorescein complex, is a fluorescent dye with excitation and emission wavelengths of 495 and 515 nm, respectively, and has the appearance of orange crystals.

Calcein AM is a cell-permeant dye frequently used in cell biology research. It is derived from Calcein, a fluorescent dye that exhibits bright green fluorescence upon binding to intracellular free calcium ions.

Calcein AM staining is a commonly used technique for live cell imaging, utilizing the non-fluorescent acetoxymethyl (AM) ester form of Calcein. This compound readily crosses live cell membranes due to its neutral charge.

Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

Calcein-AM is a cell-permeable esterase substrate that produces the highly fluorescent dye calcein after intracellular esterase hydrolysis in live cells.

Sep 13, 2024 · Calcein-AM is a green fluorogenic dye that stains live cells for cell viability in flow cytometry and fluorescence microscopy. It is the acetoxymethyl (AM) ester form of the fluorescent probe, calcein.

In this review, we elucidate the key application of calcein as a powerful yet simple tool to investigate the endosomal escape. Various conditions have been reported, which reflects the complexity of the calcein release assay and sometimes might even cause contradictive outcomes.

The calcein AM (calcein-acetoxymethyl ester) assay is a widely used fluorescent technique for monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance.

Calcein, a 29 kDa calcium-binding protein of the EF-hand family, is expressed in various normal and tumour tissues. Using immunohistochemistry, Anandani et al. found positive calcein expression in 16 ameloblastoma samples.