Feb 6, 2025 · Here, we show that engineered crRNA with a covalently closed circular structure (C-crRNA) can replace traditional linear crRNA to form functional complexes with Cas12a protein, …

Jan 11, 2025 · This 'splice-at-will' crRNA engineering strategy could faithfully detect ultrashort RNA sequences as short as 6-8 nt, which cannot be achieved by conventional Cas12a and …

Sep 30, 2020 · In this study, we engineer crRNAs with high sensitivity and specificity for detecting nucleic acids using CRISPR-Cas12a.

Feb 27, 2024 · We apply the adaptability of Cas12a:crRNA complexes to detect SARS-CoV-2 in clinical nasopharyngeal swabs, saliva samples, and tracheal aspirates. Our findings further …

Feb 2, 2025 · Overall, the diversified and integrated design of the SPACE strategy enabled simultaneous, discriminative, and low-threshold detection of dual MS-related miRNAs in one …

Jan 20, 2025 · Herein, we presented a universal ‘splice-at-will’ CRISPR RNA (crRNA) engineering mechanism that enables Cas12a to directly detect RNA, particularly the ultrashort ones with …

May 7, 2024 · In this work, we report a CRISPR-Cas12a crRNA engineering strategy by incorporating 2-aminoadenine (Z) into crRNA (Z-crRNA) to improve the binding affinity and …

Aug 10, 2022 · Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a, which exhibits excellent target DNA-activated trans -cleavage activity under the guidance of a …

Mar 19, 2025 · This study presents a novel CRISPR/Cas12a-based method for the direct and sensitive detection of miRNA-21 using split crRNA. The system comprises Cas12a protein, …

In this review, we aim to provide a mechanistic insight into the working mechanism of Cas12a, comparing it with Cas9, and eventually provide an overview of its current applications in …