Cre-Lox recombination involves the targeting of a specific sequence of DNA and splicing it with the help of an enzyme called Cre recombinase. Cre-Lox recombination is commonly used to circumvent embryonic lethality caused by systemic inactivation of many genes.

Unlock the power of the Cre/lox system for tissue-specific, inducible knockouts and advanced gene tracking in mouse models. Learn practical breeding schemes to simplify complexity, refine gene expression control, and avoid lethal phenotypes.

A various tissue-specific Cre-driver mouse lines have been generated to date, and new Cre lines are still being developed. This review provides a brief overview of Cre- loxP system and a few commonly used promoters for expression of tissue-specific Cre recombinase.

Describes mouse models for ocular research available at The Jackson Laboratory and provides a list of known mouse mutations that affect vision.

Cre activity is restricted to adult striated muscle fibers and embryonic striated muscle cells of the somites and heart. Transgenic cre activity, directed by the mouse aquaporin 2 promoter, is observed in kidney cells (collecting duct, left) and testes (sperm, right).

Cre is currently the most commonly used recombinase. It catalyzes site-specific recombination of DNA between loxP sites, thus making possible conditional mutagenesis, where specific genes can be knocked out in particular tissues and at particular developmental time points.

Cre mice, also known as Cre-loxP mice, are genetically modified mice that have become an essential tool in biomedical research. These mice have been instrumental in understanding the functions of specific genes in various biological processes.

Apr 4, 2025 · The Cre-Lox system is a powerful tool in mouse genetics, enabling precise spatiotemporal control of gene expression and conditional knockout models. Since its development, it has transformed genome editing by facilitating targeted deletions, translocations, inversions, and complex modifications—double-floxed inverse orientation. Its utility extends beyond mice to rats, pigs, and zebrafish.

Here we describe how tamoxifen-dependent Cre recombinases, so-called CreER recombinases, work and how they can be used to generate time- and tissue-specific mouse mutants. The focus will be on the CreER (T2) recombinase, which is currently the most successful CreER version.

Oct 20, 2020 · The Cre-Lox system enables gene expression with exceptional spatial (expresses a gene in specific cells, tissues, or entire organisms) and temporal (at specific time points through the application of the Cre-Lox system) control.