Cre recombinase (Cre) is able to recombine specific sequences of DNA without the need for cofactors. The enzyme recognizes 34 base pair DNA sequences called loxP ("locus of crossover in phage P1").

As of 2019, the live colony of Stock No. 007001 has been backcrossed a total of at least 11 generations (the last seven backcross generations were onto C57BL/6J).

CreERT由三个关键字Cre、ER、T组合而成，分别对应于 CreERT 系统中的三个关键物质： Cre 重组酶、雌激素受体（ER，estrogen receptor）和药物他莫昔芬（T，Tamoxifen）。 CreERT系统可被药物他莫昔芬诱导开启。 我们一一来拆解CreERT名字里的关键因素。 Cre是一种识别特异位点的基因重组酶，Cre酶的基因序列来源于P1噬菌体的环化重组酶（c yclization re combinase）， Cre 的名字也源于此。 Cre酶可识别一段名为Loxp的特定基因序列。 Loxp序列长34个碱基，包 …

008463 B6.129-<i>Gt (ROSA)26Sor^{tm1 (cre/ERT2)Tyj}</i>/J This strain enables temporal control of floxed gene expression by tamoxifen induction <i>in vivo</i>.

The second generation of inducible Cre-ERT recombinase, hemizygous B6.129S-Tg (UBC-cre/ERT2)1Ejb/J (hereafter abbreviated as Cre-ERT2), a fusion of a mutated estrogen receptor and Cre recombinase, was engineered to be more efficient and specific than the original Cre-ERT.

Sep 17, 2020 · In addition to establishing optimal dose and administration of TAM, our study reveals a disparate activity of Cre in different cell immune populations when using Cre/ER models. Tamoxifen...

This chimeric protein, called Cre‐ERT, was further modified to increase its sensitivity by the insertion of three different mutations in the hER‐LBD sequence (namely G400V, M543A, and L544A), leading to the generation of the highly ligand‐dependent Cre‐ERT2 recombinase protein, 19 which is 10 times as sensitive to 4‐OHT than Cre‐ER ...

Recent development of the tamoxifen-inducible Cre-ER T2 offers both cell type-specific and temporal control of conditional gene inactivation.

016261 C57BL/6-Tg (Nes-cre/ERT2)KEisc/J <i>Nestin-Cre/ERT2</i> transgenic mice express tamoxifen-inducible Cre recombinase in adult and developing mouse brain, including neuronal and glial cell precursors.

We have developed a conditional and inducible DNA recombination system for cell populations defined by the simultaneous activity of two promoters. The ERT2 tamoxifen binding domain of CreERT2 was added to the split-Cre system to achieve tamoxifen inducibility of split-Cre mediated DNA recombination. This strategy was named split-CreERT2.