The second generation of inducible Cre-ERT recombinase, hemizygous B6.129S-Tg (UBC-cre/ERT2)1Ejb/J (hereafter abbreviated as Cre-ERT2), a fusion of a mutated estrogen receptor and Cre recombinase, was engineered to be more efficient and specific than the original Cre-ERT.

Mar 7, 2018 · 将人雌激素受体（estrogen receptor，简称ER）的配体结合区（LBD）与Cre 重组酶相融合，形成定位于胞浆中的融合蛋白（Cre-ER）。 只有在雌激素诱导后，融合的 Cre 蛋白才会通过构象变化从锚定蛋白 HSP90上解离下来，进入细胞核，识别loxP 位点并发生重组。 这样就通过控制雌激素的注射时间，可以实现对基因重组时间特异性的调控。 为了避免内源雌激素的干扰，在配体结合区做一个点突变（G521R）就可以使 Cre-ER 只响应外源的人工合成雌激素（比 …

Sep 17, 2020 · In addition to establishing optimal dose and administration of TAM, our study reveals a disparate activity of Cre in different cell immune populations when using Cre/ER models. Tamoxifen...

CreERT由三个关键字Cre、ER、T组合而成，分别对应于 CreERT 系统中的三个关键物质： Cre 重组酶、雌激素受体（ER，estrogen receptor）和药物他莫昔芬（T，Tamoxifen）。 CreERT系统可被药物他莫昔芬诱导开启。 我们一一来拆解CreERT名字里的关键因素。 Cre是一种识别特异位点的基因重组酶，Cre酶的基因序列来源于P1噬菌体的环化重组酶（c yclization re combinase）， Cre 的名字也源于此。 Cre酶可识别一段名为Loxp的特定基因序列。 Loxp序列长34个碱基，包 …

Dec 15, 2023 · The focus of this study was to evaluate and characterize the effectiveness of different ages of Tam induction in a Cre/ERT2 mouse model. A microglial NuTRAP mouse model was used to compare induction efficiency and specificity, and to capture age-related changes in the microglial translatome.

The second generation of inducible Cre-ERT recombinase, hemizygous B6.129S-Tg (UBC-cre/ERT2)1Ejb/J (hereafter abbreviated as Cre-ERT2), a fusion of a mutated estrogen receptor and Cre recombinase, was engineered to be more efficient and specific than the original Cre-ERT.

We examined the use of ERT2-iCre-ERT2 (Cre2ERT2), a tamoxifen-regulated form of Cre that has been described to have a background activity lower than that of other tamoxifen-regulated Cre constructs, for establishing performant conditional deleter mouse lines.

As of 2019, the live colony of Stock No. 007001 has been backcrossed a total of at least 11 generations (the last seven backcross generations were onto C57BL/6J).

We have developed a conditional and inducible DNA recombination system for cell populations defined by the simultaneous activity of two promoters. The ERT2 tamoxifen binding domain of CreERT2 was added to the split-Cre system to achieve tamoxifen inducibility of split-Cre mediated DNA recombination. This strategy was named split-CreERT2.

Apr 14, 2016 · Mutant Estrogen Receptor (ERT2) ligand-binding domain fusions with Cre recombinase are a key tool for spatio-temporally controlled genetic recombination with the Cre/lox system.