Principle of Cre-DIO system - GeneMedi
Principle of Cre-DIO (double-floxed inverse open reading frame) system. Without Cre, the GOI and reporter gene are inverted and silenced. In the presence of Cre, first, the transgene will be …
A Practical Guide to Optimizing AAV DIO and FLEx Vector
Nov 2, 2021 · Learn about the sources of spontaneous recombination and optimizing Cre-recombinase dependent AAV vector expression in vivo.
基因的时间开关——CreERT系统 - 知乎
为了克服Cre酶重组过程可逆的弱点，科学家设计了另外一种常用的基因开关序列， DIO序列 （double-floxed inverted open reading frame）。 DIO序列中包含两种类型的Cre酶识别位点， …
Plasmids 101: FLEx Vectors - Addgene
Apr 28, 2016 · Sometimes also referred to as DiO (D ouble-floxed i nverse O rientation), FLEx systems are generally considered "Cre-On" meaning that your gene of interest starts in the …
Cre-loxp system and Viral vector (AAV and adenovirus)-based Cre …
Cre-loxp system is powerful tool for tissue-specific Knockout (KO) and overexpression in vivo and in vitro. Cre-loxP system is widely used in the field of biosciences, especially in the generation …
A rapid in vitro method to flip back the double-floxed inverted …
Aug 31, 2018 · The LoxP site based genetic switch, the FLEX, also known as DIO (Double-Floxed Inverted Open reading frame), was invented to turn on gene expression via Cre-mediated …
Systematic optimization and prediction of cre recombinase for …
Apr 4, 2025 · Background The Cre-Lox system is a powerful tool in mouse genetics, enabling precise spatiotemporal control of gene expression and conditional knockout models. Since its …
Off-Target Expression of Cre-Dependent Adeno-Associated …
Double inverted open reading frame (DIO) constructs are a common method to achieve Cre-dependent activation of genes. DIO constructs rely on two pairs of recombination-incompatible …
Cre-inducible Adeno-associated Virus: DIO-AAV - SignaGen
Oct 9, 2015 · For cell type-specific targeting and to capitalize on the large number of Cre driver lines with the flexible virus injection/fiberoptic approach, we have developed a tool we call DIO …
DIO-SPOTlight Transgenic Mouse to Functionally Monitor Protein …
Jan 23, 2025 · Here, we expand on the capabilities of SPOTlight by developing it in a Cre-dependent format utilizing Double-floxed Inverted Orientation (DIO) of loxP recombination …