Jan 11, 2012 · CHO cells had good viability, grew rapidly, were easy to culture and had the ability to reach high expression levels, all of these factors made them an excellent choice as a “manufacturing” cell line. Of course, early biopharmaceutical manufacturing using CHO cells was much different than production today.

Jul 21, 2011 · CHO cells had been used for many years in laboratory work beginning in 1919 for typing pneumococci. Their use declined after domestication problems led to hereditary diseases due to imbreeding. Then in 1957, Dr. Theodore T. Puck from the University of Colorado’s Department of Medicine first established ovary cells in culture plates and found ...

Jun 3, 2013 · CHO-DG44 cells were derived from the original CHO-K1 cells by several rounds of mutagenesis that deleted both copies of the dhfr genes. Dihydrofolate reductase (dhfr) is required for the de novo synthesis of purines, thymidylic acid, and certain amino acids and is required for growth and proliferation of CHO cells.

CHO cells became popular because they have a low chromosome number and were used to study genetics. As a result CHO cells have been grown for a long time and much is known about their genetics and growth characteristics. Additionally CHO cells so not express the EGR receptor so the cell line was used in reconstitution studies to demonstrate the ...

Mar 9, 2021 · We use CHO cells because because of their history and selection systems, but in terms of productivity each CHO cell is only about 10% as effective as a professional protein secretory cell. He explained that a professional protein secretory cell would be something like a plasma cell making antibodies in the human body in response to infection.

CHO-DG44 cells were derived from the original CHO-K1 cells by several rounds of mutagenesis that deleted both copies of the dhfr genes. Dihydrofolate reductase (dhfr) is required for the de novo synthesis of purines, thymidylic acid, and certain amino acids and is required for growth and proliferation of CHO cells.

Mar 5, 2019 · Figure 4: CHO-S cells were seeded the day before transfection at 1×106 cells/mL in a Mobius® single-use 3 L bioreactor (Merck) with 1.5 L of FectoCHO™ CD Expression Medium. The culture parameters were set as follows : 130 rpm, pH 7.1, 37°C, 40% pO2. 24 hours after seeding, cells were transfected with FectoPRO® (0.8 µg DNA/mL) following ...

Oct 16, 2015 · Secondly, since CHO cells are used to manufacture >70% of current licensed therapeutic proteins today, the ability to start drug development work in CHO cells and stay in CHO cells from discovery through bioproduction reduces risk, as transient CHO-derived proteins are more similar in terms of critical quality attributes to proteins made in ...

Oct 27, 2015 · CHO cells are the predominant host for biotherapeutic protein expression, with roughly 70% of licensed biologics manufactured in CHO. Multiple attributes make CHO cells desirable for bioproduction including the ability to adapt to high-density suspension culture in serum-free and chemically-defined media and the incorporation of post ...

Aug 1, 2016 · Simplifying Transient Production in CHO Cells. Recently, Irvine Scientific launched their BalanCD® Transfectory™ CHO system of medium and supplements for transient transfection in CHO cells. The system provides a scalable platform approach to transient transfection CHO with milligram to gram-scale yields of recombinant proteins.