Here, we describe a novel open-source ImageJ/Fiji tool, called ‘Quanty-cFOS’, with an easy-to-use, streamlined pipeline for the automated or semi-automated counting of cells positive for the Fos protein and/or c-fos mRNA on images derived from tissue sections.

Sep 23, 2022 · After washing, the slices were counterstained by 4’, 6-diamidino-2-phenylindole (DAPI). Images were acquired using a Nikon confocal microscope (ECLIPSE Ti2-U, Nikon, Japan).

Feb 23, 2023 · Here, we describe a novel open-source ImageJ/Fiji tool, called ‘Quanty-cFOS’, with an easy-to-use, streamlined pipeline for the automated or semi-automated counting of cells positive for the Fos protein and/or c-fos mRNA on images derived from tissue sections.

Our results suggest that cFOS staining may provide a reliable and sensitive biomarker for hippocampal formation activity development, particularly in regard to the attainment of a normal waking state and synchronizing rhythms such as theta and gamma.

Oct 26, 2022 · The mechanism preventing repeated cFos expression in DG granule cells involves accumulation of ΔFosB, a long-lived splice variant of FosB. CA1 neurons, in contrast, repeatedly expressed cFos.

Jul 28, 2020 · We created SimpylCellCounter (SCC), an efficient and accurate automated method to quantify cFos-IR cells in brain tissue. SCC utilizes binary thresholding and morphological functions from the...

Feb 6, 2025 · Essentially we have 3 different channels: blue (dapi), green (D2), red (c-Fos). We are looking to count the number of cells expressing dapi, count the amount of puncti in the green channel and eventually count how many cells are D2 positive, count the amount of puncti in the red channel and find out how many cells are c-Fos positive, and ...

Jun 3, 2019 · The number of eYFP-positive, c-Fos-positive, and DAPI-positive cells in a set region of interest (0.5 mmˆ2 per brain area analyzed) were quantified with ImageJ (https://imagej.nih.gov/ij/) and averaged within each animal.

Aug 24, 2017 · The cell body layers of dCA1, dSub, EC5, MB, or basolateral amygdala (BLA) were outlined as regions of interest (ROIs) and the percentage of cFos + neurons were calculated as ( (cFos +) / (Total DAPI +)) × 100.

Mar 20, 2023 · The goal is the compare the cfos localization with the dapi and finally see if they are “in/part of” the tuj/neuronal body, thereby technically verifying that this is indeed neuronal activation.