In urothelial carcinoma (UC), CDK7 expression is increased in bladder cancer tissues, especially in patients with chemoresistance. CDK7 inhibition-related cancer stemness suppression is a potential therapeutic strategy for both chemonaïve and chemoresistant UC.

CDK7 is both a CDK-activating kinase (CAK) and a component of the general transcription factor TFIIH. An intricate network of cyclin-dependent kinases (CDKs) is organized in a pathway to ensure that each cell accurately replicates its DNA and segregates it equally between the two daughter cells. [1] .

Mar 30, 2025 · CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression.

Oct 15, 2024 · Cyclin-dependent kinase 7 (CDK7) is the catalytic subunit of CDK-activating kinase (CAK), which phosphorylates CDK1/2/4/6 and RNA polymerase II (Pol II) to promote cell cycle progression and gene transcription, respectively. CDK7 plays critical roles in development and homeostasis. CDK7 is overexpressed in a variety of cancers.

Aug 3, 2024 · Here we report the crystal structure of the human Cdk7/Cyclin H/Mat1 complex containing both T-loop phosphorylations. Whereas pT170 coordinates basic residues conserved in other CDKs, pS164...

Cdk7, associated with the transcription initiation factor TFIIH, is both an effector CDK that phosphorylates Pol II and other targets within the transcriptional machinery, and a CDK-activating kinase (CAK) for at least one other essential CDK involved in transcription.

Jul 19, 2004 · Major changes between CDK7 and CDK2 occur in three regions: (i) the activation segment (CDK7 residues 155–174); (ii) the region where three parts of chain pack together comprising αD (CDK7 residues 101–112), the β7/β8 loop (CDK7 residues 146–149), and the C-terminal region (CDK7 residues 297–311); and (iii) the region of the L14 loop ...

Jun 6, 2018 · CDK7 activity from Jurkat T-cell immunoprecipitates or recombinant CDK7 (rCDK7) was measured in radioactive in vitro kinase assays reactions using a His-tagged RNA polymerase II CTD as substrate with or without a 15-min pre-treatment with 50 n m THZ1.

CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression.

CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression.