Aug 3, 2024 · To determine if Cdk7 S164 phosphorylation influences the formation of ternary complexes with Mat1, we established a surface plasmon resonance (SPR) spectroscopy assay.

Here we report the crystal structure of the human Cdk7/Cyclin H/Mat1 complex containing both T-loop phosphorylations. Whereas pT170 coordi-nates basic residues conserved in other CDKs, pS164...

Feb 14, 2024 · Cdk7 forms an active complex upon association with Cyclin H and Mat1, and its catalytic activity is regulated by two phosphorylations in the activation segment (T loop): the canonical activating modification at T170 and another at S164. Here we report .

Cdk7, associated with the transcription initiation factor TFIIH, is both an effector CDK that phosphorylates Pol II and other targets within the transcriptional machinery, and a CDK-activating kinase (CAK) for at least one other essential CDK involved in transcription.

Cyclin-dependent kinase 7 (CDK7) engages tumor growth by acting as a direct link between the regulation of transcription and the cell cycle. Here, we investigated the clinical significance of CDK7 expression and its potential as a therapeutic target in epithelial ovarian cancer (EOC).

Cyclin-dependent kinase 7, or cell division protein kinase 7, is an enzyme that in humans is encoded by the CDK7 gene. [5] The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family.

Jul 1, 2019 · In summary, we describe SY-5609, an orally available, potent, and selective CDK7 inhibitor that drives strong responses in CDX and PDX tumor models. These data support the rationale for advancing SY-5609 into IND-enabling studies.

Nov 2, 2021 · Cyclin-dependent kinase 7 (CDK7) is a serine-threonine kinase that plays key roles in both transcription and regulation of the cell cycle.

Cdk7, associated with the transcription initiation factor TFIIH, is both an effector CDK that phosphorylates Pol II and other targets within the transcriptional machinery, and a CDK-activating kinase (CAK) for at least one other essential CDK involved in transcription.

Feb 14, 2024 · Five different nanobodies were identified and characterized by surface plasmon resonance (SPR) spectroscopy for their binding to Cdk7/Cyclin H/Mat1 230-309, showing affinities between 0.6 and 45 nM and recognition of two different epitopes by different nanobodies (Supplementary Fig. 2a,b).