22 hours ago · Use MDM2 and CDK4 coexpression to help diagnose low grade osteosarcomas and high grade osteosarcomas that progressed from low grade osteosarcomas (Am J Surg Pathol 2010;34:1361)

Fluorescent in situ hybridization (FISH) for amplification of MDM2 has been considered the gold standard for diagnosis in these situations. Immunostaining for MDM2 and/or CDK4 has been adopted as a surrogate method because of its high concordance rate with FISH and lower cost.

The MDM2 gene and its neighboring gene CDK4 are amplified, which can be detected by molecular methods such as reverse transcription–polymerase chain reaction (RT-PCR) and FISH. The resultant MDM2 and CDK4 protein overexpression can be detected by IHC.

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Many studies have shown MDM2 and CDK4 expression by IHC to have good sensitivity and specificity in differentiating low grade osteosarcoma from benign mimics in long bones, with up to 97.5% sensitivity and 100% specificity [4, 7, 9, 10, 16, 17].

To analyze the utility and limitations of ancillary techniques, we studied 11 cases of DDLPS in challenging conditions and 17 cases of nonlipogenic high-grade sarcomas with immunohistochemistry (IHC) for p16, CDK4, and MDM2 and automated dual-color in situ hybridization (DISH) for MDM2 amplification.

The immunohistochemical trio of CDK4, MDM2, and p16 is a useful ancillary diagnostic tool that provides strong support in distinguishing WDLs and DDLs from other adipocytic neoplasms and is potentially more sensitive than previously assessed combinations of CDK4 and MDM2. p16 was the most sensitive and specific marker for detecting WDL/DDL, and ...

Cyclin dependent kinase-4 (CDK4) encoded by CDK gene, is a heterodimer protein of cell cycle in G1-S transition. This study aimed to characterize the CDK4 immunoreactivity in different histological grades of oral leukoplakias (OLs) and oral squamous ...

Aim: To investigate whether immunohistochemistry (IHC) for MDM2 and CDK4 can be used to diagnose WDLPS and DDLPS. Methods: IHC for MDM2/CDK4 was carried out on a series of 129 paraffin-embedded lipomatous and non-lipomatous soft tissue tumours.

Aug 14, 2014 · Immunohistochemical (IHC) staining for CDK4 was performed on representative paraffin-embedded tumor material from the biopsy cut into 4 µm-thick sections and placed onto glass slides. For IHC staining, the slides were deparaffinized in xylene.