Cyclin-dependent kinase 4 (CDK4), also known as cell division protein kinase 4, is an enzyme that is encoded by the CDK4 gene in humans. CDK4 is a member of the cyclin-dependent kinase family, a group of serine/threonine kinases which regulate the cell cycle. [4]

This review discusses the role that CDK4 plays in cell cycle control, normal development and tumorigenesis as well as the current status and utility of approved small molecule CDK4/6 inhibitors that are currently being used as cancer therapeutics. Keywords: CDK4/6, cancer, cell cycle, targeted therapy, checkpoint inhibitor.

Although CDK4-null mutant mice are viable and cell proliferation is not significantly affected in vitro due to compensatory roles played by other CDKs, this gene plays a key role in mammalian development and cancer.

Zuo et al. (1996) determined that the CDK4 gene contains 8 exons and spans 5 kb. Demetrick et al. (1994) mapped the CDK4 gene to chromosome 12q13 by fluorescence in situ hybridization. CDK2 maps to the same band.

Mar 30, 2025 · Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition.

In this overview, we address various molecular features of CDK4 activation that are critical but remain poorly known or debated, including the regulation of its association with D-type cyclins, its subcellular location, its activating Thr172-phosphorylation and the roles of Cip/Kip CDK "inhibitors" in these processes.

Sep 17, 2021 · In brief, CDK4 v.39 and v.40 share the new alternative splicing event between exons 1 and 6, while CDK4 v.41 involves the splicing of exon 1 with exon 7 and CDK4 v.42 is produced from the direct splicing of exon 1 with the last exon of the gene, exon 8.

Jan 1, 2007 · CDK4 is a relatively compact gene that spans 4.16 kb of genomic DNA on the long arm of chromosome 12, in the telomere-to-centromere orientation. The gene consists of eight exons of which the first exon is non-coding.

Jan 17, 2020 · Methods: We investigated the RNA gene expression profile of epidermal growth factor receptor (EGFR), cyclin-dependent kinase 4 (CDK4), murine doble minute 4 (MDM4), and platelet derived growth factor receptor alpha (PDGFRA) in 83 primary GBM tumors vs. 42 normal brain tissue samples.

Mar 6, 2025 · To elucidate their differential activities, molecular mechanisms, and inhibitor binding, we combine biochemical assays and molecular dynamics (MD) simulations. We discover that CDK4 and CDK6 have distinct allosteric networks connecting the β3-αC loop and the G-loop.