The p21-Activated kinase 1 (PAK1), a member of serine-threonine kinases family, was initially identified as an interactor of the Rho GTPases RAC1 and CDC42, which affect a wide range of processes associated with cell motility, survival, metabolism, ...

Oct 9, 2023 · In this study, we show that SARS-CoV-2 cell entry promotes ACE2 endocytosis and degradation. The ACE2-spike complex is internalized by clathrin-mediated endocytosis and P21-activated kinase 1...

Jun 9, 2022 · Here, we show that alchemical free energy calculations can quantitatively compute the effect of mutations at the protein–protein interface. As a test case, we have used the protein complex formed by the small Rho-GTPase CDC42 and its downstream effector PAK1, a serine/threonine kinase.

Dec 15, 2002 · Our results show that Cdc42/Rac1-dependent activation of PAK is necessary for lamellae spreading. Furthermore, the cortical actin-binding protein, cortactin that contributes to actin polymerization, is under the control of PAK activation.

Our data here establish that activated Cdc42 interacts with PAK1 in a nucleotide-dependent manner in the cell periphery, leading to Thr-423 phosphorylation of PAK1, particularly along the lengths of cell protrusion structures.

Apr 15, 2023 · We find that loss of Cdc42 activation enables precocious Rho1 activation in early anaphase. Furthermore, we provide functional and genetic evidence that Cdc42-dependent Rho1 inhibition is mediated by the Cdc42 target Pak1 kinase.

Aug 4, 2000 · The p21-activated kinases (PAKs), stimulated by binding with GTP-liganded forms of Cdc42 or Rac, modulate cytoskeletal actin assembly and activate MAP-kinase pathways.

Oct 15, 1999 · The Rho family small GTPase Cdc42 transmits divergent intracellular signals through multiple effector proteins to elicit cellular responses such as cytoskeletal reorganization. Potential effectors of Cdc42 implicated in mediating its cytoskeletal effect in mammalian cells include PAK1, WASP, and IQGAP1.

In this study, we showed that increased Cdc42 and PAK1 expression can decrease the expression of STMN1 and increase the expression of phosphorylated stathmin, which is located in the nucleus of PGCCs and progeny cells to regulate cytoskeletal remodeling.

Aug 1, 2020 · The p21-Activated kinase 1 (PAK1), a member of serine-threonine kinases family, was initially identified as an interactor of the Rho GTPases RAC1 and CDC42, which affect a wide range of processes associated with cell motility, survival, metabolism, cell cycle, proliferation, transformation, stress, …