Forward- and side-scatter parameters were used to gate live cells. The mean fluorescence intensity (MFI) for different markers was compared with that of negative control (extract-untreated...

The FMO for CD86 and for BTLA were tested showing MFI < 140 (CD86) and < 380 (BTLA) for all B cell subtypes. The settings for forward scatter (FSC) and side scatter (SSC) were optimized to achieve the best separation for lymphocytes, beads and trash.

Jan 1, 2018 · However, DCreg expressed comparatively low levels (MFI) of HLA-DR and CD86, but much higher levels of PD-L1 compared with imDC (Fig. 2 B). An important prerequisite of DCreg generated for therapeutic use, is the ability to …

Mar 8, 2002 · The FACScan revealed the presence of CD80 (MFI of 106.49) and CD86 (MFI of 155.80) molecules on B cells incubated with LPS for 48 h (Fig. 1 a). We also evaluated the expression of CD80, CD86, and CD40 molecules on the B cell lymphoma, WEHI-279.

Jan 29, 2014 · On the other hand, IFN-β but not IFN-α significantly enhanced CD86 B lymphocyte expression (MFI) in both HCs and HAM/TSP patients. Upon IFN-β stimulation, the induction of CD86 expression (MFI) was comparable in both groups, namely HCs (1.9 fold) and HAM/TSP patients (2.2 fold).

Luteolin impeded CD86 levels and promoted CD206 levels in M1 macrophages in a concentration-dependent pattern. The analysis represents the MFI. Flow charts of CD86 MFI in each group (a).

They exhibited similar, low levels of costimulatory CD80 and CD86, but comparatively high levels of co-inhibitory programed death ligand-1 (PD-L1) and IL-10 production compared to control immature DC (iDC).

Using flow cytometry, activation status of myeloid cells, particularly antigen-presenting cells (APCs), were assessed by quantifying the expression level of CD80, CD86 and MHC-II at the...

Mar 20, 2020 · a CD86 expression plotted as mean fluorescence intensity (MFI) with boxplots, for B cell subtypes: naïve, pre-plasmablasts (pre-PB), plasmablasts (PB), transitional B cells (TB), switched memory cells (SM), non-switched memory cells (NSM), and …

3 days ago · Mean fluorescence intensity (MFI) was also used to evaluate expression levels of CD11c, HLA-DR, and CD86. As shown in Fig. 4, celecoxib treated DCs showed high expression level of CD11c and HLA-DR, while there was no significant expression level of …