PD-1 agonism by anti-CD80 inhibits T cell activation and ... - Nature
Feb 10, 2022 · By generating anti-CD80 antibodies that detach CD80 from the cis -PD-L1–CD80 duplex and enable PD-L1 to engage PD-1 in the presence of CD80, we demonstrate that the targeted...
PD-1/CD80 + small extracellular vesicles from immunocytes
May 8, 2024 · Here we demonstrate that PD-1 and CD80 carried on immunocyte-derived sEVs (I-sEV) induce an adaptive redistribution of PD-L1 in tumour cells. The resulting decreased cell membrane PD-L1...
Restriction of PD-1 function by cis -PD-L1/CD80 interactions is
Apr 18, 2019 · Here we demonstrate that CD80 interacts with PD-L1 in cis on antigen-presenting cells (APCs) to disrupt PD-L1/PD-1 binding. Subsequently, PD-L1 cannot engage PD-1 to inhibit T cell activation when APCs express substantial amounts of CD80.
PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory
Dec 17, 2019 · Combined immunotherapy targeting the immune checkpoint receptors cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and programmed cell death 1 (PD-1), or CTLA-4 and the PD-1 ligand (PD-L1) exhibits superior anti-tumor responses compared with …
PD-L1-CD80 interactions are required for intracellular signaling
Jan 29, 2025 · We show that cis interactions between PD-L1 and CD80 are critical for promoting migration and define specific regions within these proteins necessary for migration. Furthermore, we demonstrate that αPD-L1 significantly impedes DC migration in a B16 melanoma tumor model.
Restriction of PD-1 function by cis -PD-L1/CD80 interactions is ...
May 10, 2019 · Here we demonstrate that CD80 interacts with PD-L1 in cis on antigen-presenting cells (APCs) to disrupt PD-L1/PD-1 binding. Subsequently, PD-L1 cannot engage PD-1 to inhibit T cell activation when APCs express substantial amounts of CD80.
PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory …
Cis-CD80 blocks PD-L1:PD-1 interaction and PD-1 signaling. Cis-PD-L1 inhibits CD80:CTLA-4 interaction by disrupting CD80 homodimers. Cis-PD-L1 protects CD80 from CTLA-4-mediated trans-endocytosis. Anti-PD-L1, but not anti-PD-1, depletes CD80 on APCs in a CTLA-4-dependent manner. ACKNOWLEDGMENTS
Jan 29, 2025 · In this study, we find that both preclinical murine and clinically available human PD- L1 antibodies limit DC migration. We show that cis interactions between PD- L1 and CD80 are critical for promoting migration and define specific regions within these proteins necessary for …
PD-1 agonism by anti-CD80 inhibits T cell activation and
By generating anti-CD80 antibodies that detach CD80 from the cis-PD-L1-CD80 duplex and enable PD-L1 to engage PD-1 in the presence of CD80, we demonstrate that the targeted dissociation of cis-PD-L1-CD80 duplex elicits PD-1 function in the condition where PD-1 function is otherwise restricted.
Catch and release: freeing up PD-L1 ameliorates autoimmunity
Feb 21, 2022 · Therapeutic blockade of the cis-PD-L1–CD80 interaction liberates PD-L1 to bind to PD-1, inhibits autoreactive T cells and robustly alleviates autoimmune symptoms.