During the acute phase of bleomycin-induced lung injury, the expression of CD64 and markers of “classically activated” or “M1-like” macrophages (CD40, CD80, and CD86) were increased in both alveolar and interstitial macrophages (Figures 3B and E5).

CD64: CD64, also known as FcγRI, is a high-affinity receptor for the Fc region of immunoglobulin G (IgG) molecules. CD64 expression is upregulated on M1 macrophages in response to inflammatory stimuli such as lipopolysaccharide (LPS) and interferon-gamma (IFN-γ).

In 2000, the first description of the successful use of CD64 as an immunotherapeutic target for the elimination of macrophages was demonstrated by Thepen and colleagues in a mouse model of cutaneous inflammation using CD64 monoclonal antibody (H22) chemically conjugated to the plant enzyme Ricin-A.

CD80 is the ligand for the proteins CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation) found on the surface of T-cells. [6] [13] Interaction of CD80 with CD28 triggers costimulatory signals and results in enhanced and sustained T-cell activation. In contrast, contrary ...

CD80, also known as B7, B7.1, or BB1, is a T lymphocyte activated antigen. It has a molecular weight of 60 kD. It synergizes with CD86 to activate T lymphocytes and plays an important role in autoimmune monitoring, humoral immune response and transplantation response.

The co-stimulatory molecule CD80 showed no detectable expression by THP-1 macrophages, but LPS, LPS/IFN-γ, IL-4, IC/IL-1β and IL-10 all increased expression to above the pre-determined threshold. Similarly, HLA-DR expression was not detectable by resting macrophages, but addition of IFN-γ and LPS resulted in a significant increase.

M1 and M2 were identified as CD64 + CD80 + and CD11b + CD209 +, respectively, by flow cytometry. Polarized M1 cells secreted IP-10, IFN-γ, IL-8, TNF-α, IL-1β, and RANTES, whereas M2 cells secreted IL-13, CCL17, and CCL18. Functionally, M2 cells were highly endocytic.

Dec 12, 2024 · CD64 is a high-affinity Fc receptor and CD80 binds to CD28. CD64-expressing K562 cells were loaded with anti-CD3 antibody, as described previously, 38 to activate Tregs via the TCR.

In the present study, the expression of CD64, CD40, CD163, CD206, HLA-DR, CD80 and CD86 on monocytes and the expression of CD64 on monocyte subsets were determined by flow cytometry. The expression of CD64 on monocyte subsets in patients with RA was further analyzed for their correlation with markers of autoimmune response, inflammation ...

Jan 31, 2012 · This study validated CD80 as the most robust phenotypic marker for human MΦ(IFN-γ), whereas CD200R was upregulated and CD14 was specifically downregulated on MΦ(IL-4). CD163 and CD16 were found to be specific markers for MΦ(IL-10).