pCMV-CD63-EGFP是碧云天自行研发的一种可以在哺乳动物细胞中通过CMV启动子表达人源CD63-EGFP融合蛋白的的质粒。 本产品可用于外泌体的示踪研究，用于研究外泌体的形成、分泌、靶向和运输机制。

In this study, a Cre-dependent CD63 flag -EGFP co-expressed with mCherry protein system expressing mice was constructed, which can be used for the secretion, movement, and sorting of EVs from specific tissues in vivo.

Plasmid CD63-pEGFP C2 from Dr. Paul Luzio's lab contains the insert CD63 molecule and is published in Unpublished This plasmid is available through Addgene.

Apr 29, 2020 · We demonstrate that the mutated pHluorin-CD63, pHluo_M153R-CD63, can now be expressed as a stable construct in cells and is a bright reporter for exosome secretion.

Jul 19, 2021 · Our results demonstrate that both CD63 and CD9 can be released in small ectosomes formed at the plasma membrane, and that in HeLa cells, exosomes represent a minor subpopulation of small EVs...

In this study, CD63-eGFP–expressing HEK293T cells were utilized as the donor cell line for EV production due to their common usage in therapeutic development. The isolated fluorescent EVs were co-cultured with recipient cell lines including neural and endothelial cells.

Nov 17, 2023 · 将MHCK7-CD63-EGFP-AAV载体局部注射到小鼠双侧后肢的骨骼肌中（图2A），发现小梁骨、皮质骨和生长板中的骨髓细胞亚群呈EGFP阳性，此外在皮质骨的微血管中也检测到荧光信号（图2B），表明Mu-EV可能通过血液循环直接转移到骨骼中。 随后采用DiR标记的Mu-EV通过尾静脉注射到循环中，发现注射后1、6和24h在小鼠骨骼中检测到荧光信号，表明Mu-EV可以转运到骨组织中（图2C）。 进一步将MHCK7-CD63-EGFP-AAV载体转染的骨骼肌 …

Sep 12, 2019 · To explore in situ cell-type-specific ILV/exosome labeling in the CNS, we generated Cre-dependent ILV/exosome reporter (CD63-GFP f/f) mice by inserting a loxP-floxed stop codon upstream of...

FLouEVs are a next generation of stably-fluorescent small EVs/Exosomes expressing the fluorescent protein EGFP (green) as fusion protein with the EV membrane marker CD63.

FLUO-EVs are stably-fluorescent Extracellular vesicles expressing the fluorescent protein mCherry (red) as fusion protein with tetraspanins CD9, CD81 and CD63. FLUO-EVs are purified in combination of Tangential Flow Filtration (TFF) and Size Exclusion Chromatography (SEC) from genetically engineered cells in order to express the fluorescent ...