Jun 12, 2023 · We obtained the adenocarcinomic human alveolar basal epithelial cell line A549 (ATCC CCL-185) from the American Type Culture Collection (ATCC, Manassas, VA, USA). The cells were cultivated in a CO 2 incubator (Memmert, Schwabach, Germany) under a 5% CO 2 atmosphere at 37 °C.

Apr 9, 2021 · The isolated particles from c-Jun-KO A549 cells were confirmed to be exosomes, by examining the expression of the exosome markers including CD81, CD63 and CD9 (Figure 3C). Confocal images in Figure 3D showed that Exo-c-Jun-KO was uptaken by HUVECs.

Dec 10, 2024 · Common exosomal protein markers, such as CD9 and CD63, were identified in A549 cell-derived exosomes through Western blot and flow cytometry.

In this study, we investigated the platinum nanoparticle-mediated effects on the cellular and exosomal functions of human lung epithelial adenocarcinoma cells (A549) cells cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% EVs-depleted serum and in serum-free Opti-MEM.

Feb 1, 2023 · The lung cancer (A549)-derived exosomes were isolated using ultracentrifugation techniques, and the as-obtained exosomes were characterized based on TEM, NTA, DLS, and different molecular endpoints (TSG101, CD63, and CD81) determined using Western blot.

The expression of exosomal markers including CD9 and CD63 was detected by Western blot analysis. After THP-1 cells were treated with 100 ng/mL phorbol ester (PMA) for 48 hours, the macrophage marker of CD68 was detected by real-time quantitative PCR (RT-qPCR).

Starting with cells grown in Exo-FBS (our exosome-depleted FBS) to maximize purity, exosomes are isolated using ExoQuick-TC® and then quality checked by Western blot for the presence of CD63, and NanoSight for particle size and intactness.

Dec 10, 2024 · This integrative study provides insights into the molecular interactome of lung adenocarcinoma A549 cell-derived exosomes, providing a foundation for future research on exosomal cargo and its role in tumor cell communication, growth, and progression.

We examined three exosome standards from Novus Biologicals (Table 1) derived from human plasma, human serum and A549 cells, which are adenocarcinomic human alveolar basal epithelial cells. Using these standards, we validated antibodies to common exosome targets listed in Table 2.

The miRNA expression profiles of A549 cells, A549/DDP cells and their respective exosomes Fold difference histogram (Figure 2A and 2B ) showed the differential distribution of signals in all detected gene probes (control probe and flagged probe excluded).