Aug 29, 2017 · Rab3a induced the degradation of CD63, so 293T cells co-transfected using the CD63 WT-GFP and Rab3a WT-HA expression plasmids were treated simultaneously with the lysosomal and proteasomal inhibitors to confirm the binding of CD63 and Rab3a.

Sep 20, 2016 · The levels of CD9 and CD63 protein in 293T cells and exosomes were quantified by Western blot analysis using antibodies against CD9 (H-110) (Santa Cruz, sc-9148) and CD63 (H-193) (Santa Cruz, sc-15363).

CD63, a member of the tetraspanin family, is involved in virion production by human immunodeficiency virus type 1 (HIV-1), but its mechanism is unknown. In this study, we showed that a small...

Apr 3, 2018 · To boost exosome production by increasing exosome biogenesis and secretion, we first conducted a screen in HEK-293T cells to find genes that enhance exosome production.

Dec 1, 2023 · The strategy, termed “CD63-snorkel (CD63-SNKL)”, permits anti-tag based affinity isolation. We found that CD63-SNKL fusion protein has similar subcellular localization as endogenously-expressed CD63 and faithfully sorted into Exo.

In this study, CD63-eGFP–expressing HEK293T cells were utilized as the donor cell line for EV production due to their common usage in therapeutic development. The isolated fluorescent EVs were co-cultured with recipient cell lines including neural and endothelial cells.

These data indicated CD63 and p41Gag did not interact and confirmed the specificity of the fluorescence complementation between p41Gag and Anx2. The neuronal damage characteristic of...

Apr 7, 2025 · Analysis of c HEK293T and d hADSC exosome size distribution and particle number by NanoSight. e Characterization of exosomes using western blotting with representative surface markers (CD63, CD81 ...

a) 293T‐derived exosomes were detected with antibodies against CD63 and β‐actin by immunoblotting. Left: 293T cells. Right: engineered exosomes. b) TEM image of the engineered exosomes. Scale...

May 16, 2024 · The incorporation of ARRDC1 and CD63 significantly enhanced HEK293T-sEV biogenesis, evidenced by significant increases in sEV-associated proteins TSG101 and LAMP1, resulting in a boost in sEV production.