CD63 is a good marker for flow cytometric quantification of in vitro activated basophils for diagnosis of IgE-mediated allergy. The test is commonly designated as basophil activation test (BAT).

Oct 13, 2011 · We report that siRNA depletion of CD63 from human umbilical vein endothelial cells (HUVEC) results in loss of P-selectin dependent recruitment of THP-1 monocytic cells under flow in vitro. CD63 knockout mice showed both a loss of P-selectin dependent leukocyte rolling and a failure of leukocyte extravasation in a peritonitis model.

Oct 15, 1996 · The data suggest that CD63 Ab binding to the neutrophil surface triggers a transient activation signal that requires extracellular calcium and regulates the adhesive activity of CD11/CD18. Associated protein kinase activity may play a role in signal transduction by CD63 to regulate other cell functions.

Jun 17, 2024 · Here we report that a key component of small extracellular vesicles, the tetraspanin CD63, sorts cholesterol to ILVs, generating a pool that can be mobilized by the NPC1/2 complex, and exported...

Intracellular trafficking of CD63 is connected to granule‐derived secretory pathways in human eosinophils. Eosinophil activation leads to secretion of presynthesized, granule‐stored mediators that determine the course of allergic, inflammatory, and immunoregulatory responses.

In this study, we describe new and topologically distinct CD63 scaffolds that enable robust and flexible surface engineering of the exosome. In particular, we conducted sequential deletions of the transmembrane helix of CD63 to generate a series of CD63 truncates, each genetically-fused to a fluorescent protein.

4 days ago · CD63 pathway from host multivesicular bodies provides essential lipids to Chlamydia to maintain productive intracellular infection (J Cell Sci 2006;119:350) Uses by pathologists Marker of melanoma (although non-specific) and melanocytic tumors

Aug 6, 2021 · In this study, we identify tetraspanin CD63 as a novel functional marker of HSCs in mice. We show that CD63 is unevenly expressed on the cell surface in HSC populations.

Photoaffinity labeling identified the tetraspanin CD63 as the main EP9-binding protein, which was supported by CD63-EP9 modeling data. Expression of CD63 was significantly upregulated in infarct-activated fibroblasts of mice and humans.