Apr 1, 2002 · Our results indicate that a functional CD16 molecule is a necessary and sufficient requisite for CD38 to control an activation pathway, which includes calcium fluxes, tyrosine phosphorylation of ZAP70 and mitogen-activated protein kinase, secretion of interferon-γ, and cytotoxic responses.

Feb 8, 2022 · CD16 KI combined with CD38 KO leads to superior antimyeloma activity of NK cells in vivo. We next explored whether the superior in vitro DARA-mediated ADCC of CD38 KO /CD16 KI NK cells would translate into an augemented antitumor effect in vivo.

Jun 19, 2024 · In this study, anti-CD38 targeted therapy rapidly boosted platelet levels by inhibiting antibody-dependent cell-mediated cytotoxicity on platelets, maintained long-term efficacy by clearing...

Therefore, we sought to develop an efficient CRISPR/Cas9-based gene editing platform to disrupt CD38 expression (CD38 knockout (KO)) in ex vivo expanded NK cells and simultaneously arm CD38 KO NK cells with a high-affinity CD16 (CD16-158V) receptor.

Apr 1, 2002 · Our results indicate that a functional CD16 molecule is a necessary and sufficient requisite for CD38 to control an activation pathway, which includes calcium fluxes, tyrosine phosphorylation of ZAP70 and mitogen-activated protein kinase, secretion of interferon-gamma, and cytotoxic responses.

In CD16 + NK cells, the association with CD16 is instrumental for the CD38-mediated intracellular signaling events. The high level of Ca 2+ induces ZAP70 tyrosine phosphorylation, MAPK activation, and IFN-γ secretion.

CD38 on leukocytes attaching to CD16 on endothelial cells allows for leukocyte binding to blood vessel walls, and the passage of leukocytes through blood vessel walls. [11] CD16 is often used as an additional marker to reliably identify different subsets of human immune cells. [12] .

CD38 is a multifunctional cell surface protein endowed with receptor/enzymatic functions. The protein is generally expressed at low/intermediate levels on hematological tissues and some solid tumors, scoring the highest levels on plasma cells (PC) and PC-derived neoplasia.

CD38 is expressed on membrane rafts where it promotes cell signaling via AKT and ERK activation and it is exported out of the cells through the exocytic pathway. CD38 association with the signaling complex CD16/CD61 in the NK cell membrane has a critical role in transducing activating signals.

In normal and tumor cells of B lineage, CD38 was found to be also associated with surface Ig (sIg) and with the complement receptor 2 (CR2)/CD19 complex. The CD38 molecule expressed by purified natural killer cells displayed an association with the low affinity IgG Fc receptor (Fc gamma RIII) CD16.