CD34 - 110kDa glycosylated transmembrane protein; it is a marker of vascular endothelial cells and a sensitive but not specific marker for neoangiogenesis

Immunohistochemistry is an integral component in the proper analysis of soft tissue tumours, and a simple panel of six markers is useful in practical triage: CD34, desmin, epithelial membrane antigen (EMA), keratin cocktail AE1/AE3, S100 protein and alpha smooth muscle actin (SMA).

In patients with CD34-positive blasts, the CD34 IHC can improve the detection of residual blasts on Day 14 bone marrow biopsy in comparison with morphological assessment of blast percentage in bone marrow aspirate. Keywords: Acute myeloid leukemia; early response.

CD34 is commonly used as a marker to identify and isolate CSCs in various types of cancer. CD34-positive CSCs have been identified in leukemia, breast cancer, lung cancer, and other types of tumors. These CD34-positive CSCs have been shown to have enhanced self-renewal ability and increased tumor-initiating capacity compared to CD34-negative cells.

CD34 is a transmembrane phosphoglycoprotein, first identified on hematopoietic stem and progenitor cells. Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants.

CD34 is expressed in hematopoietic progenitor cells and endothelial cells of blood vessels. Thus, it has been used as a marker for capillaries and blood vessels. One of the most densely vascular organs is the kidney, wherein networks of capillaries are intertwined with renal tubules.

Dec 21, 2024 · Investigation of bone marrow (BM) trephine biopsies and/or clot sections by CD34 immunohistochemistry (IHC) testing has been used by pathologists for several decades, and its clinical value has been well established with QBEND10 being the most frequently used primary antibody (Ab) clone.

Review of the published literature resulted in 49 articles relevant to clinical applications of the BM CD34 IHC. Five key questions were addressed including testing indications, technical performance, readout methodology, terminology, and reporting.

CD34 (Endothelial Cell Marker) is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains.

Apr 23, 2020 · In this study, we assessed the percentage of CD34+ blasts by IHC in BM biopsies from MDS patients and compared the results to the blast counts obtained by conventional CM and, in a subset of patients, also to the enumeration of CD34+ myeloid blast cells by FCM.