Nov 16, 2008 · Since the antigenic properties of the circulating CD34 cells may yield clues to disease pathogenesis and have not been extensively studied, we used five-color flow cytometry to examine these cells from 20 well characterized patients with PMF and 10 normal controls.

Since the small population of cells that bear the CD34 antigen are thought to be responsible for multilineage engraftment, graft assessment by flow cytometric quantitation of CD34+ cells should provide a rapid, reliable, and reproducible assay.

Flow cytometric testing of the peripheral blood is extremely sensitive method for enumerating CD34 + cells and can detect fewer than one CD34 + cell/µL.

CD34 - 110kDa glycosylated transmembrane protein; it is a marker of vascular endothelial cells and a sensitive but not specific marker for neoangiogenesis

CD34 + progenitor cells were quantified by flow cytometry using the International Society of Hemotherapy and Graft Engineering (ISHAGE) protocol. 23 PB samples for CD34 + cell counts were collected from each patient immediately before beginning leukapheresis.

Oct 23, 2023 · In this work, we describe the validation plan with relevance to the risk analysis to test the inter-laboratory reproducibility of the CD34+ enumeration method. All the steps from transport of samples to the final report were carefully examined adopting a failure mode and effect analysis scheme.

Jul 1, 2011 · Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells in leukapheresis product and bone marrow for clinical transplantation: a comparison of three methods.

The CD34 blood test measures the concentration of CD34+ cells in the bloodstream. This test typically involves collecting a blood sample from the patient using standard phlebotomy methods. Samples are then analyzed through established laboratory techniques, such as flow cytometry.

In this study we have used a direct true volumetric counting of CD34+ cells under continuous flow pump to overcome potential drawbacks with impact in rare cell analysis.

Oct 16, 2022 · Flow cytometry can quickly identify the abnormal cell population, characterize its phenotype, give lineage classification, make the diagnosis, or narrow down the differential list. ... CD10, CD38, TdT and CD24. CD34 is commonly expressed, but its expression is quite variable, and often dim or partial. CD45 is usually dim and can be completely ...