Jun 4, 2002 · The physiologic role of eight CD19 tyrosines was examined in CD19-knockout mice expressing transgenic CD19 constructs. CD19 Y482 and Y513 were essential for normal B cell biology, including differentiation of B1 and marginal zone B cells and for T-dependent and -independent antibody responses.

Jun 1, 2000 · The B220 + PNA high cells were determined to consist of mature B cells because they uniformly expressed the B cell lineage markers CD19, CD21/35, CD22, and CD40, excluding contamination by either non-B cells or immature B cells (Fig. 2 and Table I).

Studies of mutant forms of CD19 revealed differences in signaling in different compartments inside the germinal center. Structural findings indicate a selective role in the interaction with follicular dendritic cells in the GC.

The top portion shows flow-cytometric analysis of GC B cells (PNA hi CD38 lo) among gated CD19 + B cells of Fas fl/lpr and Fas fl/lpr Ighg1-Cre mice. Numbers are the percentage of GC B cells among total B cells.

B-lymphocyte antigen CD19, also known as CD19 molecule (Cluster of Differentiation 19), B-Lymphocyte Surface Antigen B4, T-Cell Surface Antigen Leu-12 and CVID3 is a transmembrane protein that in humans is encoded by the gene CD19.

Thus, the initial activation to the PNA + stage can be mediated by CD19-independent signaling. However, mice with mutations in CD19 Y482 and Y513 demonstrated profound defects in proliferation and differentiation of GC B cells once recruited into the FDC zone.

Feb 1, 2004 · Germinal center B cells (CD19 + PNA hi Fas hi) are boxed, with the percentage of total lymphocytes and standard deviations indicated. Gated CD19 + B cells are shown.

bodies. In these mice, GC B cell differentiation, prolif-can be identified by binding of peanut agglutinin (PNA) eration, and class switching occur but are defective. and by the fact that they are in cell cycle. The standard Altered CD19 signaling results in retention of early model of GC development is based on the identification

Jan 1, 2003 · CD19 is a 95-kDa transmembrane protein bearing two extracellular Ig domains and an extensive cytoplasmic tail (4, 5). Onset of CD19 expression occurs at the earliest stages of B cell development before the expression of the pre-BCR.

Jul 29, 2021 · GC B cells are characterized by a specific cell surface phenotype, and are usually gated as CD19 + B220 + IgD − CD38 − GL7 + CD95 + PNA + in mouse, and CD19 + IgD − CD38 int CD10 + in humans (see sections 6.2.2 Murine germinal center B cells, and 6.2.3 Human B cells and their subsets, in reference ). GCB cells also express high levels of ...