In this review we will describe the main subsets of B cells in peripheral blood and how these can be identified using flow and mass cytometry, as well as important changes in B-cell subsets in inflammatory immune mediated conditions.

Feb 17, 2021 · We have shown recently that constitutive active Notch2-signaling in B cells strongly shifts transitional type 1 (T1) B cells toward the MZB cell lineage, even in the absence of CD19 21. The...

Given that splenic MZB cells are known to express higher levels of IgM, CD19, and CD267 (TACI) and lower levels of IgD, 21, 49 we examined the expression spectra of these markers on pulmonary MZB cells.

Aug 4, 2020 · ACKR3 is required for development of a functional MZ and for positioning of MZBs. Deletion of ACKR3 on B cells distorts the MZ, and MZBs fail to deliver antigens to follicles, reducing humoral responses. Reconstitution of MZ-deficient CD19 ko mice shows that ACKR3 − MZBs can differentiate into ACKR3 + MZBs, but not vice versa. The lack of a ...

Mar 18, 2022 · They identified organ-specific differences in activated B cell populations and resolved two populations of marginal zone B cells, MZB-1 and MZB-2. These differ in tissue abundance and distribution, transcriptional profiles, immunoglobulin repertoire diversity, and frequency of SHM.

Here we show that the transcriptional repressor capicua (CIC) and its binding partner, ataxin-1-like (ATXN1L), play important roles in FOB and MZB cell development. CIC deficiency reduces the size of both FOB and MZB cell populations, whereas ATXN1L deficiency specifically affects …

Furthermore, our data show that Notch2IC-expression overcomes the CD19-dependency of MZB cell development. The newly generated MZB cells localized to the marginal zone. MZB cells reside in a well-defined splenic microenvironment, the so-called marginal zone (MZ).

Jul 14, 2017 · We found here that CD19 deficiency blocked the differentiation of marginal zone precursors (MZP) to MZ B cells, whereas CD19 expression in CD19‐deficient MZP rescues MZ B‐cell generation. Furthermore, CD19 regulates Notch2 cleavage by up‐regulating ADAM28 expression in MZP.

Dec 8, 2011 · Notch2IC-driven MZ B-cell generation in the spleen was achieved even in the absence of CD19. Our results implicate that a constitutive Notch2 signal in transitional type 1 B cells is sufficient to drive MZ B-cell differentiation.

May 1, 2003 · Here we demonstrate that Notch2 but no other Notch family member is preferentially expressed in mature B cells and that conditionally targeted deletion of Notch2 results in the defect of marginal zone B (MZB) cells and their presumed precursors, CD1d hi fraction of type 2 transitional B cells.