Administration of diphtheria toxin results in depletion of dendritic cell populations.

Nov 29, 1994 · To this end we have developed a conditional cell ablation strategy that is based on dendritic cell-restricted expression of a Diphtheria Toxin receptor (DTR) using the CD11c/Itgax promoter. Here, we provide basic protocols that describe the use of this prototypic dendritic cell ablation model and highlight pitfalls and strengths of the approach.

In this study, we find that transient depletion of CD11c + cells from CD11c.DTR mice results in the CCR2-independent, G-CSF-dependent expansion of a novel CD64 + Ly6C + monocyte population in the spleen that is transcriptionally distinct from both Ly6C + and Ly6C neg monocytes.

Feb 26, 2012 · Transgenic mice expressing the diphtheria toxin receptor (DTR) in specific cell types are key tools for functional studies in several biological systems. B6.FVB-Tg (Itgax-DTR/EGFP)57Lan/J...

Jan 16, 2013 · Dendritic cells (DCs) play a key role in regulating innate and adaptive immunity. Our understanding of DC biology has benefited from studies in CD11c.DTR and CD11c.DOG mouse models that use the CD11c promoter to express a diphtheria toxin (DT) receptor transgene to inducibly deplete CD11c + cells.

In these mice efficient DC depletion can be achieved over prolonged periods of time by multiple injections of diphtheria toxin. We show here that NK cells require DC for full acquisition of effector function in vivo in response to the bacterial-derived TLR ligand CpG.

Jul 5, 2018 · The prototype of the DTR approach, the CD11c-DTR mouse, nicely illustrates the pitfall of insufficient promoter specificity. At the time of its generation, CD11c promoter activity was widely considered to be restricted to DC 28 .

Oct 28, 2008 · In order to study the interactions between DC and NK cells in vivo we utilize here a new bacterial artificial chromosome (BAC) transgenic CD11c.DTR mouse model, designated CD11c.DOG, which allows effective depletion of DC over prolonged periods of time without non-specific cytotoxicity.

CD11c+ T-bet+ atypical B cells (ABCs) have been identified in the context of vaccination, acute and chronic infections and autoimmune disease. However, the origins and functions of ABCs remain elusive.

DT-treated CD11c-DTR transgenic mice could therefore be a valuable source of DC-depleted allografts to further investigate the role of DC in transplant survival. The present study focuses on the potential of DC to induce protective CD8 + T cell responses, i.e., cross-priming.