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    Nature
    https://www.nature.com › articles

    Targeting CCL2-CCR4 axis suppress cell migration of head and …

    • The human head and neck squamous cell carcinoma (HNSCC) cell line HSC3, HSC6 were provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA). Human HNSCC cell lines SCC15, SCC25, and CAL27, h… See more

    Human Cytokine Antibody Array

    Culture supernatant was used to analyze the cytokine profiles with the RayBiotech® Human … See more

    Nature
    Enzyme-Linked Immunosorbent Assay

    CCL2 levels were detected in the cell culture supernatant for 48 h in respective conditions and quantified using Human CCL2 ELISA Kit (Telenbiotech, Guangzhou… See more

    Nature
    Human Tissue Microarrays

    Human tissue microarrays OR208 were selected and purchased from Alenabio (Xi’an, SN, CN) for the research. Each tissue microarray contains 60 cases of human ora… See more

    Nature
    Immunohistochemistry Staining

    For IHC, the tissue sections or tissue microarrays were deparaffinized in xylene twice and rehydrated in a graded series of ethanol (100, 95, 85, and 75% ethanol) and phosphate … See more

    Nature
    Cell Migration Assay

    To detect the cell migration potential, a transwell migration assay (8-ÎĽm pore size; Corning, Corning, NY, USA) was performed. Briefly, 5 × 104 cells/well of HSC6 cell… See more

    Nature
    Is CC chemokine receptor 4 a target for cancer immunotherapy?
    PLoS One. 2014; 9 (7): e103776. CC chemokine receptor 4 (CCR4) represents a potentially important target for cancer immunotherapy due to its expression on tumor infiltrating immune cells including regulatory T cells (T reg s) and on tumor cells in several cancer types and its role in metastasis.
    ncbi.nlm.nih.gov
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