May 16, 2016 · We co-transfected both green fluorescent protein (GFP)-Pxn and Flag-Ccnd1 into rat prostate tumour cells (R3327-5′A) and performed an IP against GFP.

Significance: IIn conclusion, our study demonstrated the regulatory mechanism between CCND1 and FGFR1 in FGFR1 amplified LSCC. Co-targeting CCND1 and FGFR1 could provide greater clinical benefits. KEYWORDS: CCND1, FGFR1, CO …

Input (IN) and immunoprecipitation (IP) samples were analysed by western blot using anti-HA and anti-Flag antibodies, to detect co-IP of the C-terminus of α4 with Ccnd1.

(A) H1299 cell lysates subjected to an endogenous CCND1 IP under nondenaturing conditions using A/G beads. Precipitates probed for CCND1 to detect high molecular-weight species, presumably ubiquitylated CCND1.

May 16, 2016 · IP of endogenous Ccnd1 was carried out in MEFs with a rabbit polyclonal antibody (#06–137, Upstate, 5 μg). IP of endogenous Pxn was done in Ccnd1 −/− and Ccnd1 +/+ immortalized MEFs with an anti-Pxn rabbit polyclonal antibody (H …

Dec 2, 2016 · These findings uncover new mechanisms for MCL cells to increase CCND1 protein level through somatic mutations, which may provide a survival advantage to the lymphoma cells in developing resistance against the emerging ibrutinib therapy.

IR-based autophagy induced during the G1 and S counteracts the accelerated cell cycle in cancer and lays the groundwork for combined therapies combined with IR. It has been shown that CCND1 suppresses autophagy in mammary epithelium [85] whereas CCND1 inhibits autophagy by activating AMPK in breast cancer cells [77].

These findings indicate that CCND1 amplification may be a key point related to immunosuppression in TME and multiple malignancy hallmarks, and it hinders not only the natural host immune responses but also the efficacy of ICIs.

Key findings: CCND1 was co-overexpressed with FGFR1 in lung cancer patients. Overexpression of CCND1 promoted LSCC cell proliferation and metastasis. FGFR1 promoted the processes of EMT through AKT/MAPK signaling by targeting CCND1 in FGFR1-amplification cell lines.

Oct 2, 2018 · (A) H1299 cell lysates subjected to an endogenous CCND1 IP under nondenaturing conditions using A/G beads. Precipitates probed for CCND1 to detect high molecular-weight species, presumably ubiquitylated CCND1.