Incorporation of the thymidine analog 5′-bromo-2′-deoxyuridine (BrdU) has been established as a popular assay for determining cell proliferation rates in a wide variety of species, ranging from plants to mammalian cells (Cecchini et al. 2012, Nagar et al. 2002). One common assay set-up is to supplement the culture media of growing cells with BrdU.

Sep 19, 2023 · Le principe consiste à incuber des cellules en culture avec de la BrdU qui va s'incorporer dans l’ADN, à la place de la Thymine. Seules les cellules en cours de synthèse d'ADN sont capables de l'incorporer, ce qui permettra d’étudier la phase S du cycle cellulaire.

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical structure similar to thymidine. BrdU is commonly used to study cell proliferation in living tissues [1] and has been studied as a radiosensitizer [2] and diagnostic tool in people with cancer. [3]

We will review a generalized protocol for performing this staining using bromodeoxyuridine (BrdU, a thymidine analog that is incorporated into newly synthesized DNA strands) and propidium iodide (PI, a DNA dye that stains all DNA), followed by analysis of the stained cells with flow cytometry.

Pour étudier la prolifération cellulaire, il est possible de surveiller l'incorporation d'un isotope radioactif, la [3H]-thymidine, dans l'ADN cellulaire, puis d'effectuer une autoradiographie. La thymidine peut également être remplacée par la 5-bromo-2′-désoxyuridine (BrdU).

BrdU is a synthetic nucleoside analog, like the nucleoside thymidine, except the methyl group is replaced with a bromine atom. BrdU can be incorporated into DNA whenever thymidine normally would be, including during DNA replication and repair.

Si on attend suffisamment longtemps (quelques semaines), on peut déterminer le phénotype dans lequel les cellules-filles se différencient, en combinant le marquage immunocytochimique BrdU avec le marquage immunocytochimique d’un marqueur phénotypique (double immunocytochimie).

The BrdU Staining Kits for Flow Cytometry contains the necessary reagents and buffers for identifying and examining proliferating cells by flow cytometric analysis. Cycling cells are incubated with 5-bromo-2’- deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis.

Click-iT assays use a modified nucleoside, EdU (5-ethynyl-2´-deoxyuridine), that is incorporated during DNA synthesis and detected using a click reaction—a copper (I)-catalyzed reaction between an azide and an alkyne. After incubation, Centrifuge cells, …

La bromodésoxyuridine est un nucléoside synthétique, analogue structurel de la thymidine, où le groupe méthyle en 5 est remplacé par un atome de brome. Cette molécule est utilisée pour déterminer la phase du cycle cellulaire d'une cellule et notamment si cette dernière est en phase de réplication de l' ADN.