Apr 24, 2018 · Here, we describe a detailed step-by-step protocol to assay the proliferative capacity of adult hemocytes by BrdU incorporation and PH3 expression in response to high dose of bacterial infection. Methods

Loss of APC/C results in a loss of mitotic progression and apoptosis; in this study, co-labeling with BrdU and pH3 revealed cells undergoing mitotic catastrophe. In this chapter, we provide protocols to characterize the various phases of cell division in zebrafish embryos, and protocols to detect DNA damage, senescence and cell death.

Apr 24, 2018 · Here, we describe a detailed step-by-step protocol to assay the proliferative capacity of adult hemocytes by BrdU incorporation and PH3 expression in response to high dose of bacterial infection.

测S 期检验点通常采用BrdU 或EdU 方法。BrdU (5-Bromo-2ʹ-Deoxyuridine) 全称5-溴-2'-脱氧尿嘧啶核苷，EdU(5-Ethynyl-2ʹ-Deoxyuridine) 全称5-乙炔基-2'-脱氧尿嘧啶 核苷，它们均为胸腺嘧啶类似物，能够在S 期替代胸腺嘧啶 (T) 掺入正在复制的DNA 中。

The most common technique used to label progenitor cells in the brain is the thymidine analogue bromodeoxyuridine (BrdU) assay. It is the primary tool for study of the cell proliferation and cell fate.

Feb 1, 2013 · S-to-M progression was quantified by co-labeling for BrdU (red) and PH3 (green; arrows) or Ki67 (48-hour group, arrows). M-phase cells that did not incorporate BrdU were labeled as PH3+/BrdU negative (arrowheads).

In control animals, bromodeoxyuridine-labelled cells (green, A, C) were found in the ventricular zone (VZ) and subventricular zone (SVZ). A number of the bromodeoxyuridine-positive cells...

Apr 24, 2018 · Drosophila and mammalian hematopoiesis share several similarities that ranges from phases to the battery of transcription factors and signaling molecules that execute this process.

Download scientific diagram | Schema and transverse sections showing BrdU incorporations combined with PH3 in the pallium of S. canicula. a BrdU was administrated to embryos at S30 of...

Jan 1, 2004 · In zebrafish, the phospho-histone H3 antibody (pH3) stains mitotic cells throughout the embryo (Fig. 1A). In developing organs like the nervous system, pH3 staining increases as zebrafish embryos undergo proliferation during distinct developmental stages.