Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical structure similar to thymidine. BrdU is commonly used to study cell proliferation in living tissues [1] and has been studied as a radiosensitizer [2] and diagnostic tool in people with cancer.

The synthetic halogenated pyrimidine analog, 5-bromo-2′-deoxyuridine (BrdU), is a marker of DNA synthesis. This exogenous nucleoside has generated important insights into the cellular mechanisms of the central nervous system development in a variety ...

Jan 30, 2023 · During the BrdU assay, BrdU is incorporated into replicating DNA and can be detected using anti-BrdU antibodies. EdU staining is an alternative method with a shorter, simpler protocol than that required for BrdU. BrdU labeling can be done both in vitro and in vivo.

Step-by-step protocol for the use of BrdU (5-Bromo-2´-Deoxyuridine) to detect DNA synthesis using a fluorescence microscope.

The focus is on the patterns of unscheduled DNA synthesis (UDS) linked to ongoing UVC-induced DNA repair. The research underlines the insights gained through super-resolution microscopy.

Apr 22, 2019 · Using this method, we detect 5-bromodeoxyuridine (BrdU) incorporated by Saccharomyces cerevisiae to reveal, at a genomic scale and on single molecules, the DNA sequences replicated during a...

BrdU is a synthetic nucleoside analog, like the nucleoside thymidine, except the methyl group is replaced with a bromine atom. BrdU can be incorporated into DNA whenever thymidine normally would be, including during DNA replication and repair.

BrdU is a synthetic nucleoside analog, like the nucleoside thymidine, except the methyl group is replaced with a bromine atom. BrdU can be incorporated into DNA whenever thymidine normally would be, including during DNA replication and repair.

Bromo-deoxyuridine (BrdU) incorporation into replicating DNA is a commonly used and highly reproducible method to detect cell division history. Here, we describe a protocol for BrdU incorporation analysis in hematopoietic stem and progenitor cells that can provide a sensitive measure of cell division even in rare cell populations.

dU staining Introduction BrdU labeling can be performed in vitro for cell lines and primary cell cultures, or in vivo for labeling cell. within a living animal. BrdU is incorporated into replicating DNA and can be detected us.