Cell proliferation can be measured with the thymidine analog BrdU (5-bromo-2’-deoxyuridine) following its incorporation into newly synthesized DNA and its subsequent detection with an anti-BrdU antibody.

Jan 30, 2023 · BrdU (bromodeoxyuridine or 5-bromo-2'-deoxyuridine) is an analog of the nucleoside thymidine used in the BrdU assay to identify proliferating cells. BrdU labeling can be performed in vitro for cell lines and primary cell cultures …

Incorporation of the thymidine analog 5′-bromo-2′-deoxyuridine (BrdU) has been established as a popular assay for determining cell proliferation rates in a wide variety of species, ranging from plants to mammalian cells (Cecchini et al. 2012, Nagar et al. 2002).

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical structure similar to thymidine. BrdU is commonly used to study cell proliferation in living tissues [1] and has been studied as a radiosensitizer [2] and diagnostic tool in people with cancer. [3]

Mar 26, 2025 · BrdU Cell Proliferation Assay kit detects BrdU incorporation into cellular DNA during cell proliferation. The BrdU-labeled DNA has to be denatured to be detected by the BrdU Mouse mAb used in this kit.

Cycling cells are incubated with 5-bromo-2’- deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, the cells are stained for BrdU incorporation along with any other cell surface and/or intracellular targets of interest.

BrdU is a synthetic nucleoside analog, like the nucleoside thymidine, except the methyl group is replaced with a bromine atom. BrdU can be incorporated into DNA whenever thymidine normally would be, including during DNA replication and repair.

Aug 22, 2024 · BrdU is a synthetic nucleoside analog, like the nucleoside thymidine, except the methyl group is replaced with a bromine atom. BrdU can be incorporated into DNA whenever thymidine normally would be, including during DNA replication and repair.

The most common technique used to label progenitor cells in the brain is the thymidine analogue bromodeoxyuridine (BrdU) assay. It is the primary tool for study of the cell proliferation and cell fate.

BrdU staining protocol Introduction BrdU labeling can be performed in vitro for cell lines and primary cell cultures, or in vivo for labeling cells within a living animal. BrdU is incorporated into replicating DNA and can be detected using anti-BrdU antibodies. After BrdU labeling, an additional DNA hydrolysis step (sometimes referred to as a