This standard operating procedure (SOP) pertains to processing samples for Biolayer interferometry (BLI) analysis using the Octet R RH16 instrument (Sartorius) and Octet R Discovery software (v13.0).

Oct 24, 2024 · ntroduction Biolayer Interferometry (BLI) is an optical technique that measures macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a bi. sensor tip. BLI experiments are used to …

Design and development of biological assays that conform to USP <1032> requires parameters for accuracy, precision, linearity and limit of quantitation (LOQ) to be established. This document is intended as a general guideline for developing product-specific ligand binding quantitation assays on Octet® R series instruments.

BLI is an optical analytical technique that analyzes the interference pattern of white light reflected from two surfaces. Changes in the number of molecules bound to the biosensor causes a shift in the interference pattern that is measured in real time.

In BLI, the binding between a ligand immobilized on the biosensor tip and an analyte in solution produces an increase in optical thickness at the biosensor tip, resulting in a wavelength shift proportional to the extent of binding (Azmiri and Lee, 2015; Mechaly et al., 2016).

Bio-layer interferometry measures kinetics and biomolecular interactions on a basis of wave interference. To prepare for BLI analysis between two unique biomolecules, the ligand is first immobilized onto a bio compatible biosensor while the analyte is in solution. [5] .

Dec 10, 2024 · Collection and Analysis Shutdown Data Management Introduction Biolayer Interferometry (BLI) is an optical technique that measures macromolecular interactions by analyzing interference patterns. of white light reflected from the surface of a biosensor tip. BLI experiments are used to .

BioLayer Interferometry (BLI) is an optical technique for measuring macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. BLI experiments are used to determine the kinetics and affinity of molecular interactions.

Octet® Bio-Layer Interferometry (BLI) label-free binding technology is an ideal tool for small-molecule drug development research that helps accelerate candidate discovery and selection timelines.

Biolayer Interferometry (BLI) is a relatively new optical technique that analyzes macromolecular interactions in real-time, producing more high-throughput information than previously designed technologies.