The transfection potency of lipid nanoparticle (LNP) mRNA systems is critically dependent on the ionizable cationic lipid component. LNP mRNA systems composed of optimized ionizable lipids often display distinctive mRNA-rich "bleb" structures.

May 12, 2023 · The results presented here show that bleb structures can be induced in LNP mRNA systems by preparing in the presence of high concentrations of pH 4 buffer and that these systems exhibit enhanced transfection properties both in vitro and in vivo. There are three areas that warrant further discussion.

May 17, 2024 · Although lipid nanoparticles (LNPs) have been FDA-approved for mRNA delivery, there is still much to learn about these fascinating multi-component delivery systems. Here, I discuss the presence of “bleb” structures on LNPs and the co-existence of mRNA-empty LNPs in LNP-mRNA-based formulations.

Sep 1, 2024 · Here, I discuss the presence of “bleb” structures on LNPs and the co-existence of mRNA-empty LNPs in LNP-mRNA-based formulations.

Recognizing that mRNA can dissociate from the charged lipid to reside within a solvent-filled bleb compartment has direct implications for the chemical microenvironment of the mRNA and confers a new significance to this liposomal-like LNP structural domain.

Although lipid nanoparticles (LNPs) have been FDA-approved for mRNA delivery, there is still much to learn about these fascinating multi-component delivery systems. Here, I discuss the presence of "bleb" structures on LNPs and the co-existence of mRNA-empty LNPs in LNP-mRNA-based formulations.

Feb 1, 2025 · Notably, significant differences emerged in the prevalence of LNPs with bleb structures (bleb LNPs). The T-junction microreactor produced a higher proportion of bleb LNPs, associated with slightly enhanced mRNA integrity, while the ring microreactor predominantly formed spherical LNPs.

Jul 5, 2024 · Here, we reformulate lipid nanoparticles (LNPs) through adjustments in lipid material structures and compositions to systematically achieve the pulmonary and hepatic (respectively) targeted...

Jan 16, 2024 · We show evidence that SAM changes its conformational structure when encapsulated in LNPs, becoming more compact than the free SAM form. A characteristic "bleb" structure is observed in SAM LNPs, which consists of a lipid-rich core and an aqueous RNA-rich core, both surrounded by a DSPC-rich lipid shell.

Sep 1, 2024 · Here, I discuss the presence of “bleb” structures on LNPs and the co-existence of mRNA-empty LNPs in LNP-mRNA-based formulations. Specifically, I discuss key articles on these structural and compositional heterogeneities, whether these features present negative or positive LNP attributes, and how to deal with them in research and quality ...